Use of coupled open-tubular capillaries for in-line ion-exchange preconcentration of anions by capillary electrochromatography with elution by a transient isotachophoretic gradient

Journal of Chromatography. a
Joseph P HutchinsonPaul R Haddad

Abstract

Open-tubular capillaries have been joined together for use in on-column ion-exchange preconcentration of anions by capillary electrochromatography (CEC) with elution by a transient isotachophoretic gradient. This involved the coupling of a preconcentration capillary and a separation capillary using a PTFE sleeve. Such coupling allowed precise lengths of differently coated capillaries to be joined in-line to form a single multi-mode column. The different segments could be tailored to optimize a separation by either altering the length of each segment to precisely manipulate the amount of stationary phase present or by changing the internal diameter of each segment to alter the phase ratio in the chromatographic column without affecting the path length for UV detection. In this work, a segmented in-line capillary was used in conjunction with a fluoride-octanesulfonate discontinuous electrolyte system to increase the number of anions that could be preconcentrated and separated. Quaternary ammonium functionalised latex particles were used for creating the preconcentration segment and the separation segment was coated with poly(diallyldimethylammonium chloride). This allowed the detection of trace anions in drinking water and in sit...Continue Reading

References

Nov 24, 1995·Journal of Chromatography. a·M A StrausbauchJ P Landers
Aug 29, 2000·Journal of Chromatography. a·E Rapp, E Bayer

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Citations

Dec 1, 2006·Electrophoresis·Junjie OuHanfa Zou
Jul 12, 2007·Journal of Separation Science·Stuart D ChambersCharles A Lucy
Dec 7, 2007·Electrophoresis·F W Alexander TempelsGerhardus J de Jong
Oct 14, 2008·Electrophoresis·Francisco J LaraFermín Alés-Barrero
Nov 30, 2005·Electrophoresis·Ewa Dabek-Zlotorzynska, Valbona Celo
Nov 30, 2005·Electrophoresis·Sebastiaan Eeltink, Wim Th Kok
Jan 15, 2005·Analytical Chemistry·Philip ZakariaPaul R Haddad
Nov 21, 2009·Analytica Chimica Acta·Hiroyuki KataokaKeita Saito

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