Use of crosslinkers to inactivate dentin MMPs

Dental Materials : Official Publication of the Academy of Dental Materials
R Seseogullari-DirihanA Tezvergil-Mutluay

Abstract

This study evaluated the endogenous matrix metalloproteinase (MMP) activity of demineralized dentin matrix following 1 or 5min pretreatment by various collagen crosslinkers. Generic MMP activity assay, total protein analysis, in situ zymography, gelatin zymography and multiplex bead technology were used to evaluate matrix-bound MMP activity. Six different crosslinkers; glutaraldehyde, riboflavin/UVA, riboflavin-5-monophospate/UVA, sumac berry extract, grape seed extract, and curcumin were used. Demineralized dentin beams were pretreated with respective crosslinkers for 1 or 5min. Demineralized dentin beams with no crosslinker pretreatment served as control. The reduction in the total activity of dentin matrices were measured using generic MMP activity assay. Dentin slabs were used for in situ zymography and evaluated by using hydrolysis of self-quenched fluorescein-conjugated gelatin under confocal microscopy. Dentin beam extracts were used for total protein assay and multiplex analysis and powder extracts were used for gelatin zymography. MMP activity in crosslinker pretreated samples decreased significantly between 21% and 70%, whereas untreated control samples' activity increased up to 84%. Zymograms confirmed a decrease in ...Continue Reading

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Citations

Oct 18, 2016·Dental Materials : Official Publication of the Academy of Dental Materials·Roda Seseogullari-DirihanArzu Tezvergil-Mutluay
May 17, 2017·Journal of Dentistry·Xue-Qing HuangFranklin R Tay
May 13, 2017·Journal of Dental Research·A MazzoniL Breschi
Dec 22, 2017·Brazilian Dental Journal·Tânia Mara da SilvaEduardo Bresciani
Mar 2, 2018·Dental Materials Journal·Roda Seseogullari-DirihanArzu Tezvergil-Mutluay
Sep 14, 2020·Microscopy Research and Technique·Bianca Silva GomesMaristela Barbosa Portela
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Jan 20, 2021·Journal of Dentistry·Rocio Geng VivancoFernanda de Carvalho Panzeri Pires-de-Souza
Oct 29, 2020·Journal of Applied Oral Science : Revista FOB·Fabrícia CardosoHeitor Marques HonÓrio
Mar 6, 2021·Dental Materials : Official Publication of the Academy of Dental Materials·Ana P FugolinCarmem S Pfeifer
Feb 19, 2021·Journal of the Mechanical Behavior of Biomedical Materials·Paula A BaldionDiego E Betancourt
Mar 9, 2021·Advances in Pharmacological and Pharmaceutical Sciences·Eliane Avany Malveira AraújoMaria Fulgência Costa Lima Bandeira
Jun 5, 2017·Dental Materials : Official Publication of the Academy of Dental Materials·Zihou ZhangDwayne Arola
May 9, 2019·Dental Materials : Official Publication of the Academy of Dental Materials·U DaoodA S Fawzy
Feb 17, 2018·Dental Materials : Official Publication of the Academy of Dental Materials·Qianmin OuXuefeng Lin
Oct 9, 2018·Dental Materials : Official Publication of the Academy of Dental Materials·C Parise GréBart Van Meerbeek
Dec 18, 2018·Dental Materials : Official Publication of the Academy of Dental Materials·Milena CadenaroLorenzo Breschi
Aug 28, 2021·International Journal of Molecular Sciences·Tatjana MaravićAnnalisa Mazzoni
Dec 6, 2021·European Journal of Oral Sciences·Meltem Tekbas AtayArzu Tezvergil-Mutluay

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