Use of culture, PCR analysis, and DNA microarrays for detection of Campylobacter jejuni and Campylobacter coli from chicken feces

Journal of Clinical Microbiology
Georgios KeramasClaus Bo Vöge Christensen

Abstract

A DNA microarray for detection of Campylobacter spp. was recently developed and applied to detect Campylobacter spp. directly from chicken feces. Sixty-five pooled chicken cloacal swab samples from 650 individual broiler chickens were included in the study. The results of Campylobacter sp. detection obtained with DNA microarrays were compared to those obtained by conventional culture and gel electrophoresis. By conventional culture, 60% of the samples were positive for either Campylobacter jejuni or Campylobacter coli. By PCR and capillary electrophoresis, 95% of the samples were positive for Campylobacter spp., whereas with DNA microarrays all samples were positive for Campylobacter spp. By application of DNA microarray analysis, the isolates in 4 samples (6%) could not be identified to the species level, whereas by PCR-capillary electrophoresis, the isolates in 12 samples (19%) remained unidentified. Interestingly, PCR-capillary electrophoresis analysis revealed that two (3%) of the samples were positive for both C. jejuni and C. coli, while DNA microarray analysis revealed that nine (14%) of the samples were positive for both species. Of 65 samples, 2 samples were identified to contain C. coli by conventional culture but wer...Continue Reading

References

Dec 1, 1992·Applied and Environmental Microbiology·B A GiesendorfH G Niesters
Mar 1, 1992·Journal of Clinical Microbiology·S L On, B Holmes
Jan 1, 1992·International Journal of Food Microbiology·R R BeumerF M Rombouts
Jun 1, 1992·The Journal of Applied Bacteriology·G J MedemaA H Havelaar
Sep 1, 1991·Journal of Clinical Microbiology·S L On, B Holmes
Sep 1, 1987·American Journal of Epidemiology·M S DemingR O Sikes
Apr 1, 1986·American Journal of Public Health·N V HarrisC M Nolan
Feb 1, 1995·The Journal of Veterinary Medical Science·R ItohJ Yatsuyanagi
Nov 1, 1994·Letters in Applied Microbiology·R Kirk, M T Rowe
Nov 1, 1996·Letters in Applied Microbiology·H N RasmussenO F Rasmussen
Aug 1, 1997·Molecular and Cellular Probes·D K WintersM F Slavik
Oct 26, 1999·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·D N Fredricks, D A Relman
Aug 29, 2003·Molecular and Cellular Probes·Georgios KeramasClaus Bo Vöge Christensen

❮ Previous
Next ❯

Citations

Oct 2, 2007·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·S BurteauJ Remacle
Apr 10, 2007·Applied and Environmental Microbiology·Beatriz QuiñonesRobert E Mandrell
Jan 30, 2007·Journal of Microbiological Methods·Dong WeiAleksandr L Simonian
Mar 21, 2007·International Journal of Food Microbiology·Tom HumphreyMogens Madsen
Aug 30, 2005·Clinica Chimica Acta; International Journal of Clinical Chemistry·Alexander Loy, Levente Bodrossy
Jan 7, 2018·Applied and Environmental Microbiology·Jennifer WeidhaasRubayat Jamal
Nov 14, 2018·Critical Reviews in Food Science and Nutrition·Sarah L JonesKristen E Gibson
Feb 17, 2018·Microbiology Spectrum·Deepak Kumar, Siddhartha Thakur
May 17, 2008·Journal of Agricultural and Food Chemistry·Shu ZhuKatsuko Komatsu

❮ Previous
Next ❯

Related Concepts

Related Feeds

Campylobacteriosis (ASM)

Campylobacteriosis is caused by the bacteria Campylobacter jejuni and is a common cause of gastroenteritis in humans. Discover the latest research on Campylobacteriosis here.