PMID: 3756140Jul 29, 1986Paper

Use of cytochrome P-450scc to measure cholesterol-lipid interactions

Biochemistry
V L StevensA H Merrill

Abstract

The interaction of cholesterol with phospholipids has been studied with a variety of techniques; however, the possible consequences of such interactions in vivo have not been demonstrated. In this study, the cholesterol-dependent absorbance spectrum of cytochrome P-450scc was used to monitor cholesterol availability in both micellar and vesicular environments. By use of this approach, in conjunction with titration of putative cholesterol binding species, a tight, approximately equimolar complex of cholesterol and digitonin was demonstrated. Sphingomyelin (SM) (both the synthetic N-palmitoyl and bovine brain forms) gave sigmoidal titration curves, suggesting a cooperative interaction between this lipid and cholesterol. The interaction of bovine brain glycerolipids and cholesterol was weaker than that of SM and showed no cooperativity. The importance of the phospholipid head group in these interactions was established by the differences in the ability of synthetic 1-palmitoyl-2-oleoylphosphatidylcholine, -phosphatidylethanolamine, and -phosphatidylserine to affect cholesterol availability. Comparison of these results with those of the bovine brain phospholipids indicates that the acyl chain composition of these molecules is also ...Continue Reading

Citations

May 1, 1990·Biochimica Et Biophysica Acta·A H Merrill, D D Jones
Sep 1, 1986·Microbiological Reviews·O Käppeli
Aug 1, 1996·The Journal of Steroid Biochemistry and Molecular Biology·R C TuckeyK J Cameron
Jan 16, 2007·Molecular and Cellular Endocrinology·Karl-Heinz StorbeckAmanda C Swart

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