Use of cytology centrifuged supernatants improves cost and turnaround time for targeted next generation sequencing.

Diagnostic Cytopathology
HamzaN GokozanNavid Sadri

Abstract

Molecular testing is an essential step in providing patients with advanced non-small-cell lung cancer (NSCLC), the most appropriate front-line targeted therapies. We recently implemented targeted NGS on previously discarded cytology centrifuged supernatant (CCS). In this study, we reviewed our implementation process to evaluate its performance. Performance and turnaround time (TAT) of molecular testing on all cytology NSCLC cases submitted for targeted NGS from June 2018 to September 2019 were evaluated, which included 46 and 62 cytology cases before and after implementation of CCS, respectively. Associated cost savings using CCS was also analyzed. The mean TAT defined as the time of collection to time of reporting was 8.5 ± 1.8 days in CCS cohort (range 5-13) as compared with 12.2 ± 5.3 days in the (FFPE) cell block (CB) cohort (range: 6-27). The success rate of sequencing was similar for both cohorts (100% in CCS and 96% in FFPE CB). Our results demonstrate that NGS using CCS improves TAT, preserves FFPE CB for other testing, and results in cost savings of $50 per case.

References

Aug 28, 2013·The Journal of Clinical Endocrinology and Metabolism·Marina N NikiforovaYuri E Nikiforov
Sep 25, 2015·Cytopathology : Official Journal of the British Society for Clinical Cytology·E VigliarG Troncone
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Feb 22, 2018·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Sinchita Roy-ChowdhuriRajyalakshmi Luthra
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Dec 24, 2018·Archives of Pathology & Laboratory Medicine·Lester J LayfieldRobert L Schmidt
Mar 20, 2019·Annals of Oncology : Official Journal of the European Society for Medical Oncology·B HanniganS Roy-Chowdhuri

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