PMID: 9445025Jan 28, 1998Paper

Use of differential display reverse transcription-PCR to reveal cellular changes during stimuli that result in herpes simplex virus type 1 reactivation from latency: upregulation of immediate-early cellular response genes TIS7, interferon, and interferon regulatory factor-1

Journal of Virology
R Tal-SingerS L Berger

Abstract

The detailed mechanism which governs the choice between herpes simplex virus (HSV) latency and reactivation remains to be elucidated. It is probable that altered expression of cellular factors in sensory neurons leads to induction of HSV gene expression resulting in reactivation. As an approach to identify novel cellular genes which are activated or repressed by stimuli that reactivate HSV from latency and hence may play a role in viral reactivation, RNA from explanted trigeminal ganglia (TG) was analyzed by differential display reverse transcription-PCR (DDRT-PCR). Nearly 50 cDNAs whose mRNA level was modified by the stress of explantation were isolated and sequenced. We present a listing of a spectrum of altered RNAs, including both known and unknown sequences. Five of those differentially displayed transcripts were identified as interferon-related murine TIS7 mRNA. These results were confirmed in both infected and uninfected ganglia by quantitative RNase protection assay and immunostaining. Alpha and beta interferons and interferon regulatory factor-1 (IRF-1) were also induced by explantation. In addition, we have identified sequences that correspond to IRF-1 consensus binding sites in both HSV type 1 origins of replication....Continue Reading

References

Jan 1, 1991·Current Eye Research·N W FraserR M Gesser
Jan 1, 1991·Annual Review of Biochemistry·H R Herschman
Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Mar 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·F Tirone, E M Shooter
Jul 1, 1989·Journal of Neuroscience Research·A T ArenanderH R Herschman
Sep 1, 1989·Microbiological Reviews·J G Stevens
Jan 1, 1987·Annual Review of Microbiology·B Roizman, A E Sears
May 1, 1974·Infection and Immunity·M L CookJ G Stevens
May 25, 1982·Nucleic Acids Research·D SkupE De Maeyer
Jan 1, 1995·Journal of Cancer Research and Clinical Oncology·T TaniguchiM Lamphier
Jan 1, 1994·Progress in Nucleic Acid Research and Molecular Biology·H R HerschmanS T Reddy
Apr 1, 1994·Molecular and Cellular Biology·C E DabrowskiP A Schaffer
Jan 1, 1995·Methods in Enzymology·P LiangA B Pardee
Apr 16, 1996·Biochemical and Biophysical Research Communications·K InokuchiF Ozawa
May 1, 1996·International Journal of Radiation Biology·J YanM F Lavin

❮ Previous
Next ❯

Citations

Jun 28, 2008·Seminars in Ophthalmology·Hassanain S TomaJames M Hill
Oct 13, 2006·Current Opinion in Infectious Diseases·J D Kriesel
Sep 28, 2004·Cornea·Shiro HigakiYoshikazu Shimomura
Feb 12, 2000·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·S NoisakranD J Carr
Sep 11, 2020·International Ophthalmology·Piotr Kanclerz, Jorge L Alio
Nov 24, 1998·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·C S MillerR J Jacob
Aug 10, 2000·Neoplasia : an International Journal for Oncology Research·A JacobsC Fraefel

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.