Use of differential DNA-repair host mediated assays to investigate the biotransformation of xenobiotics in Drosophila melanogaster. I. Genotoxic effects of nitrosamines

Chemico-biological Interactions
S KnasmüllerM Kehrer

Abstract

A rapid differential DNA-repair assay procedure was developed to investigate the biotransformation of xenobiotics in Drosophila melanogaster in vivo. Indicator of genotoxic activity was a pair of streptomycin-dependent Escherichia coli strains differing vastly in DNA repair capacity (uvr+/rec+ vs. uvrB/recA). Prior to the experiments with test compounds, mixtures of the two strains were injected into the abdomina of untreated animal hosts (male Berlin-K flies) and the time-dependent recovery kinetics determined. Subsequently, different aliphatic and aromatic nitrosamines were tested. Solutions of the compounds were injected simultaneously with the indicator cells. Three hours later, the flies were killed, homogenized and the induction of (repairable) DNA damage determined by comparison of the survival rates of the two strains in single animals. Eight carcinogenic compounds (nitrosodiethylamine, NDEA; nitrosodimethylamine, NDMA; nitrosodi-npropylamine, NDPA; nitrosodiethanolamine, NDELA; nitrosomethylaniline, NMA; 4-methyl-nitrosopiperidine, MNPIP; nitrosopyrrolidine, NPYR; nitrosomorpholine, NMOR) and one whose tumorigenic activities are still controversially discussed (nitrosodiphenylamine, NDPhA) induced dose-dependent differ...Continue Reading

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Citations

Jun 1, 1994·Mutation Research·D McGregor
Jun 16, 2011·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Laura Castañeda-PartidaIrma Delfín-Alcalá

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