Use of Dried Plasma Spots for HIV-1 Viral Load Determination and Drug Resistance Genotyping in Mexican Patients

BioMed Research International
Juan Pablo Rodriguez-AuadRosalia Lira

Abstract

Monitoring antiretroviral therapy using measurements of viral load (VL) and the genotyping of resistance mutations is not routinely performed in low- to middle-income countries because of the high costs of the commercial assays that are used. The analysis of dried plasma spot (DPS) samples on filter paper may represent an alternative for resource-limited settings. Therefore, we evaluated the usefulness of analyzing DPS samples to determine VL and identify drug resistance mutations (DRM) in a group of HIV-1 patients. The VL was measured from 22 paired plasma and DPS samples. In these samples, the average VL was 4.7 log10 copies/mL in liquid plasma and 4.1 log10 copies/mL in DPS, with a correlation coefficient of R = 0.83. A 1.1 kb fragment of HIV pol could be amplified in 14/22 (63.6%) of the DPS samples and the same value was amplified in plasma samples. A collection of ten paired DPS and liquid plasma samples was evaluated for the presence of DRM; an excellent correlation was found in the identification of DRM between the paired samples. All HIV-1 pol sequences that were obtained corresponded to HIV subtype B. The analysis of DPS samples offers an attractive alternative for monitoring ARV therapy in resource-limited settings.

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Citations

Jul 5, 2012·PloS One·Martine G AabyeMorten Ruhwald
Apr 24, 2016·International Journal of Biological Macromolecules·Riccardo A A MuzzarelliAntonio Gigante
Oct 14, 2017·The Journal of Infectious Diseases·Dolly SinghDeborah Persaud

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Methods Mentioned

BETA
genotyping
PCR
nucleic acid extraction
Assay

Software Mentioned

Mega
CLC bio

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Antimicrobial Resistance (ASM)

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