Use of Electrical Penetration Graph Technology to Examine Transmission of 'Candidatus Liberibacter solanacearum' to Potato by Three Haplotypes of Potato Psyllid (Bactericera cockerelli; Hemiptera: Triozidae)

PloS One
Tariq MustafaJoseph E Munyaneza

Abstract

The potato psyllid, Bactericera cockerelli (Šulc) (Hemiptera: Triozidae), is a vector of the phloem-limited bacterium 'Candidatus Liberibacter solanacearum' (Lso), the putative causal agent of zebra chip disease of potato. Little is known about how potato psyllid transmits Lso to potato. We used electrical penetration graph (EPG) technology to compare stylet probing behaviors and efficiency of Lso transmission of three haplotypes of potato psyllid (Central, Western, Northwestern). All haplotypes exhibited the full suite of stylet behaviors identified in previous studies with this psyllid, including intercellular penetration and secretion of the stylet pathway, xylem ingestion, and phloem activities, the latter comprising salivation and ingestion. The three haplotypes exhibited similar frequency and duration of probing behaviors, with the exception of salivation into phloem, which was of higher duration by psyllids of the Western haplotype. We manipulated how long psyllids were allowed access to potato ("inoculation access period", or IAP) to examine the relationship between phloem activities and Lso transmission. Between 25 and 30% of psyllids reached and salivated into phloem at an IAP of 1 hr, increasing to almost 80% of psyl...Continue Reading

References

Apr 12, 2013·Environmental Entomology·K D SwisherJ M Crosslin
Feb 13, 2014·Environmental Entomology·Alex ArpBlake Bextine
Mar 1, 2009·Plant Disease·Lia W LieftingGerard R G Clover

Citations

Jun 9, 2016·Environmental Entomology·W Rodney CooperStephen F Garczynski
Jun 24, 2017·Annual Review of Phytopathology·Nian WangJoaquim Martins

Related Concepts

Metazoa
Electromagnetic Fields
Electrophysiology (Science)
Feeding Patterns
Homoptera
Solanum tuberosum
Proteobacteria
Genetic Vectors
Hemiptera
Potato

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