Use of Electroporation To Generate a Thiobacillus neapolitanus Carboxysome Mutant.

Applied and Environmental Microbiology
R S EnglishJ M Shively

Abstract

Two cloning vectors designed for use in Escherichia coli and the thiobacilli were constructed by combining a Thiobacillus intermedius plasmid replicon with a multicloning site, lacZ(prm1), and either a kanamycin or a streptomycin resistance gene. Conditions necessary for the introduction of DNA into T. intermedius and T. neapolitanus via electroporation were examined and optimized. By using optimal electroporation conditions, the gene encoding a carboxysome shell protein, csoS1A, was insertionally inactivated in T. neapolitanus. The mutant showed a reduced number of carboxysomes and an increased level of CO(inf2) necessary for growth.

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Citations

Aug 6, 2008·Nature Reviews. Microbiology·Todd O YeatesJessup M Shively
May 11, 2011·Photosynthesis Research·George S Espie, Matthew S Kimber
Apr 4, 2020·Molecular Biology Reports·Ibrahim Ilker Ozyigit
Jan 19, 1999·Annual Review of Microbiology·J M ShivelyW G Meijer
Mar 10, 2006·Applied Microbiology and Biotechnology·Thomas A Bobik
Oct 22, 2008·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Shouqiang ChengThomas A Bobik
Oct 10, 2013·Critical Reviews in Biotechnology·Neus Ferrer-MirallesAntonio Villaverde
Mar 12, 2004·Annual Review of Plant Physiology and Plant Molecular Biology·Aaron Kaplan, Leonora Reinhold

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