Use of electrospray ionization ion-trap tandem mass spectrometry and principal component analysis to directly distinguish monosaccharides

Rapid Communications in Mass Spectrometry : RCM
Bing XiaLisheng Ding

Abstract

Carbohydrates are good source of drugs and play important roles in metabolism processes and cellular interactions in organisms. Distinguishing monosaccharide isomers in saccharide derivates is an important and elementary work in investigating saccharides. It is important to develop a fast, simple and direct method for this purpose, which is described in this study. Stock solutions of monosaccharide with a concentration of 400 μM and sodium chloride at a concentration of 10 μM were made in water/methanol (50:50, v/v). The samples were subjected to electrospray ionization ion-trap tandem mass spectrometry (ESI-MS) and the detected [2M + Na - H(2)O](+) ions were further investigated by tandem mass spectrometry (MS/MS), followed by applying principal component analysis (PCA) on the obtained MS/MS data sets. The MS/MS spectra of the [2M + Na - H(2)O](+) ions at m/z 365 for hexoses and m/z 305 for pentoses yielded unambiguous fragment patterns, while rhamnose can be directly identified by its ESI-MS [M + Na](+) ion at m/z 187. PCA showed clustering of MS/MS data of identical monosaccharide samples obtained from different experiments. By using this method, the monosaccharide in daucosterol hydrolysate was successfully identified. A ne...Continue Reading

References

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Citations

Feb 6, 2015·Journal of the American Society for Mass Spectrometry·Gabe Nagy, Nicola L B Pohl
May 24, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Coralie AudoinGrégory Genta-Jouve
Nov 16, 2018·Journal of the American Society for Mass Spectrometry·Zachary WookeNicola L B Pohl
Jan 5, 2017·Journal of the American Society for Mass Spectrometry·Yanglan TanNicolas C Polfer

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