Use of electrospray tandem mass spectrometry for identification of microcystins during a cyanobacterial bloom event

Biochemical and Biophysical Research Communications
Humberto Vieira FriasErnani Pinto

Abstract

Drastic environmental conditions such as elevated temperature, abrupt pH variation, low turbulence, and high nutrient inputs can enhance the development of toxic cyanobacterial blooms in lakes and reservoirs. This study describes the occurrence of four microcystin variants (MC) in a bloom in the eutrophic reservoir Billings, in São Paulo City. The bloom sample was collected in October 2003, and Microcystis were the main genus found. The MC were separated and purified by reverse phase high performance liquid chromatography (RP-HPLC). Their structures were elucidated by electrospray ionization tandem mass spectrometry (ESI-MS/MS) and four MC variants were determined: MC-RR, MC-LR, MC-YR, and MC-hRhR. MC-hRhR is described for the first time as a new variant of MC with two homoarginines at positions 2 and 4 in its structure. ESI-MS/MS analysis thus provides a powerful and convenient tool for the determination of variants of MC. These results represent an important contribution to the knowledge of the biochemistry of toxic cyanobacteria and their toxins, specifically in São Paulo State.

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Citations

May 2, 2014·Journal of the American Society for Mass Spectrometry·Yulin QiDietrich A Volmer
Apr 7, 2010·Toxicon : Official Journal of the International Society on Toxinology·Felipe Augusto DörrSandra Maria Feliciano de Oliveira e Azevedo
Jun 24, 2011·Rapid Communications in Mass Spectrometry : RCM·Felipe Augusto DörrErnani Pinto
Aug 2, 2006·Toxicon : Official Journal of the International Society on Toxinology·Fabyana Maria dos AnjosErnani Pinto
Aug 12, 2006·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Karina H M CardozoErnani Pinto
Jul 31, 2013·Environment International·Sylvain MerelOlivier Thomas
Jul 5, 2012·FEMS Microbiology Ecology·Ronaldo Leal CarneiroErnani Pinto
Mar 26, 2011·Brazilian Journal of Biology = Revista Brasleira De Biologia·M C Bittencourt-OliveiraE Pinto
Jun 3, 2014·European Journal of Mass Spectrometry·Stella Bortoli, Dietrich A Volmer
Jan 31, 2007·Journal of Mass Spectrometry : JMS
Dec 11, 2019·Toxins·Noureddine BouaïchaTri Nguyen-Quang
May 19, 2007·Analytical Chemistry·Susan D Richardson

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