Use of ELISA to identify Protoceratium reticulatum as a source of yessotoxin in Norway

Toxicon : Official Journal of the International Society on Toxinology
Ingunn A SamdalChristopher O Miles

Abstract

Picked cells of Protoceratium reticulatum collected from five locations in Norway were shown by ELISA analysis to contain yessotoxins (YTXs). The production of yessotoxin (YTX) was verified by culturing followed by LC-MS analysis of one of the Norwegian isolates. This is the first report of the biogenic origin of YTXs in Norway. The sensitivity of the ELISA method made it possible to quantitate YTXs in algal cultures, net-hauls, and in single cells of P. reticulatum. The cells picked from cultures and net-hauls contained 18-79 pg YTXs per cell. Dilution series and analyses of cells from non-YTX-producing algal species demonstrated the presence of only minimal matrix effects on the ELISA, probably attributable to the presence of salts. The sensitivity of this method makes it possible to search for other possible producers of YTXs, and might also make it possible to follow the YTXs through the food chain. This method allows, for the first time, measurement of the variability in toxin content within a population of dinoflagellate cells--rather than just the average amount of toxin per cell.

References

Nov 15, 1997·Bioscience, Biotechnology, and Biochemistry·T Yasumoto, A Takizawa
Jul 10, 1999·Toxicon : Official Journal of the International Society on Toxinology·R DraisciM Pompei
Feb 27, 2001·Toxicon : Official Journal of the International Society on Toxinology·H RamstadT Aune
Aug 24, 2004·Toxicon : Official Journal of the International Society on Toxinology·Christopher O MilesA Lincoln MacKenzie
Sep 16, 2004·Journal of Agricultural and Food Chemistry·Lyn R BriggsNeale R Towers

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Citations

Aug 30, 2008·Marine Drugs·Beatriz PazJosé J Fernández
May 26, 2005·Toxicon : Official Journal of the International Society on Toxinology·Ingunn A SamdalChristopher O Miles
Mar 24, 2011·Toxicon : Official Journal of the International Society on Toxinology·John A B AasenTore Aune
Oct 15, 2010·Bioorganic & Medicinal Chemistry Letters·Satoru UjiharaYasushi Ishihama
Nov 21, 2009·Toxicon : Official Journal of the International Society on Toxinology·Humberto J DominguezJosé J Fernández
Apr 14, 2009·Bioorganic & Medicinal Chemistry Letters·Ryota MouriYasukatsu Oshima
Mar 31, 2007·Toxicon : Official Journal of the International Society on Toxinology·Beatriz PazJosé M Franco
Feb 3, 2005·Toxicon : Official Journal of the International Society on Toxinology·John AasenTore Aune
Dec 8, 2004·Toxicon : Official Journal of the International Society on Toxinology·Christopher O MilesVeronica Beuzenberg
Aug 24, 2004·Toxicon : Official Journal of the International Society on Toxinology·Christopher O MilesA Lincoln MacKenzie
Aug 22, 2006·Toxicon : Official Journal of the International Society on Toxinology·Beatriz PazJosé M Franco
Aug 24, 2006·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Mónica Suárez KorsnesTore Aune
Mar 15, 2006·Toxicon : Official Journal of the International Society on Toxinology·Christopher O MilesA Lincoln MacKenzie
Jul 16, 2013·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Mónica Suárez KorsnesChristopher O Miles
Mar 24, 2011·Toxicon : Official Journal of the International Society on Toxinology·Mónica Suárez Korsnes, Arild Espenes
Jun 15, 2011·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Toshiyuki Suzuki, Michael A Quilliam
Apr 21, 2021·Harmful Algae·Bengt KarlsonSanna Suikkanen
Jul 26, 2021·Harmful Algae·Teri L KingVera L Trainer
Dec 7, 2007·Journal of Agricultural and Food Chemistry·Jared I LoaderChristopher O Miles
Sep 16, 2004·Journal of Agricultural and Food Chemistry·Lyn R BriggsNeale R Towers

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