Use of energy-filtering transmission electron microscopy for routine ultrastructural analysis of high-pressure-frozen or chemically fixed plant cells

Protoplasma
U Lütz-Meindl, N Aichinger

Abstract

In the present study energy-filtering transmission electron microscopy by use of an in-column spectrometer is employed as a powerful tool for ultrastructural analysis of plant cells. Images of unstained very thin (50 nm) and thick (140 nm) sections of the unicellular green alga Micrasterias denticulata, as a model system for a growing plant cell, taken by conventional transmission electron microscopy are compared to those obtained from filtering at zero energy loss (elastic bright field) and to those generated by energy filtering below the carbon-specific absorption edge at about 250 eV. The results show that the high-contrast images produced by the latter technique are distinctly superior in contrast and information content to micrographs taken at conventional transmission electron microscopy mode or at elastic bright field. Post- or en bloc staining with heavy metals, which is indispensable for conventional bright-field transmission electron microscopy, can be completely omitted. Delicate structural details such as membranous or filamentous connections between organelles, organelle interactions, or vesicle and vacuole contents are clearly outlined against the cytoplasmic background. Also, immunoelectron microscopic localizati...Continue Reading

Citations

Dec 18, 2013·Journal of Plant Physiology·Stefanie VollandUrsula Lütz-Meindl
Jun 11, 2011·Plant & Cell Physiology·Patric M SchmölzerIlse Foissner
Feb 22, 2014·Apoptosis : an International Journal on Programmed Cell Death·Barbara KleinHubert H Kerschbaum
May 6, 2008·Journal of Plant Physiology·Christine LehnerUrsula Lütz-Meindl
Jun 13, 2006·Micron : the International Research and Review Journal for Microscopy·Ursula Lütz-Meindl
Dec 27, 2005·Micron : the International Research and Review Journal for Microscopy·Ursula Lütz-Meindl, Cornelius Lütz
Apr 14, 2020·Plant Methods·Othmar BuchnerUrsula Lütz-Meindl

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