Use of EPR oximetry with India ink to measure the pO2 in the liver in vivo in mice

Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine
T NakashimaH M Swartz

Abstract

The partial pressure of oxygen (pO2) of the liver in vivo in unanesthetized mice was determined using electron paramagnetic resonance (EPR) oximetry with India ink. The EPR spectra were obtained using a low-frequency (1.2 GHz) EPR spectrometer with a loop gap cavity resonator. The line width of the India ink used in this experiment was reversibly broadened by oxygen and was particularly sensitive to pO2 below 30 torr. After the administration of India ink into the tail vein, the India ink particles were taken up mainly by Kupffer cells in the liver and in part by phagocytes in the spleen. The pO2 measured in the normal liver was about 14 torr and was constant for the 2-week experimental period. The pO2 decreased when measured at 1, 2, and 6 days after treatment with hepatotoxin (carbon tetrachloride (CCl4)); within 2 weeks, it returned almost to the initial level. Measurements by EPR at sacrifice of controls and CCl4-treated mice indicated that more than 90% of the India ink went to the liver; the spleen contained 4.7% of total amount in control mice and 8.8% in CCl4-treated mice when measured 2 weeks after the treatment. These data indicate the usefulness of India ink for measuring the pO2 of the liver in vivo and that the pO2...Continue Reading

References

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Citations

Jul 11, 1998·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·B GallezH M Swartz
Nov 19, 2003·Archives of Biochemistry and Biophysics·Ramasamy P PandianPeriannan Kuppusamy
Oct 24, 2003·Free Radical Biology & Medicine·Ramasamy P PandianPeriannan Kuppusamy
Nov 1, 1996·Applied Radiation and Isotopes : Including Data, Instrumentation and Methods for Use in Agriculture, Industry and Medicine·K MäderH M Swartz
Jul 17, 2016·Radiation Protection Dosimetry·Bernard Gallez
Jan 30, 2007·Biophysical Journal·Anna BrataszPeriannan Kuppusamy
Jun 18, 2015·Angewandte Chemie·Emmanuel RoussakisConor L Evans
Aug 14, 1998·Physics in Medicine and Biology·H M Swartz, R B Clarkson

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