Use of experimental design in the investigation of stir bar sorptive extraction followed by ultra-high-performance liquid chromatography-tandem mass spectrometry for the analysis of explosives in water samples

Journal of Chromatography. a
Sébastien SchrammMaxime Bridoux

Abstract

A method for the sensitive quantification of trace amounts of organic explosives in water samples was developed by using stir bar sorptive extraction (SBSE) followed by liquid desorption and ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS). The proposed method was developed and optimized using a statistical design of experiment approach. Use of experimental designs allowed a complete study of 10 factors and 8 analytes including nitro-aromatics, amino-nitro-aromatics and nitric esters. The liquid desorption study was performed using a full factorial experimental design followed by a kinetic study. Four different variables were tested here: the liquid desorption mode (stirring or sonication), the chemical nature of the stir bar (PDMS or PDMS-PEG), the composition of the liquid desorption phase and finally, the volume of solvent used for the liquid desorption. On the other hand, the SBSE extraction study was performed using a Doehlert design. SBSE extraction conditions such as extraction time profiles, sample volume, modifier addition, and acetic acid addition were examined. After optimization of the experimental parameters, sensitivity was improved by a factor 5-30, depending on the compound stu...Continue Reading

References

Apr 6, 2000·Science & Justice : Journal of the Forensic Science Society·D WarrenK Ritchie
Oct 26, 2005·Journal of Pharmaceutical and Biomedical Analysis·Migaku KawaguchiHiroyuki Nakazawa
Feb 15, 2008·Journal of Chromatography. a·Romain TachonJean-Jacques Minet
Mar 12, 2009·Journal of Separation Science·Fernando M LancasIgor R B Olivares
Jan 16, 2013·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Fabio GosettiEmilio Marengo
Mar 20, 2013·Journal of Pharmaceutical and Biomedical Analysis·Juan Ignacio CachoManuel Hernández-Córdoba

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