Use of extracellular matrix hydrogel from human placenta to restore hair-inductive potential of dermal papilla cells

Regenerative Medicine
Xinyu ZhangZhi Qi Hu

Abstract

Aim: To explore the feasibility of human placenta extracellular matrix (HPECM) hydrogel in restoring the hair-inductive capacity of high-passaged (P8) dermal papilla cells (DPCs) for hair follicle regeneration. Materials & methods: HPECM hydrogel was prepared following decellularization and enzymatic solubilization treatment. DPCs isolated from human scalp were cultured in 2D and 3D environments. The hair-inductive ability of DPCs was assessed by quantitative RT-PCR, immunofluorescence staining, immunoblotting and patch assay. Results: DPCs (P8) formed spheres when cultured on the HPECM hydrogel. The expression levels of Versican, ALP, and β-catenin were restored in the DP spheres. HPECM hydrogel-cultured DP spheres co-grafted with newborn mouse epidermal cells regenerated new hair follicle. Conclusion: HPECM hydrogel successfully restores the hair-inductive capacity of high-passaged DPCs.

References

Oct 1, 1988·Clinics in Dermatology·R F Oliver, C A Jahoda
Sep 1, 1994·The British Journal of Dermatology·B K HandjiskiR Paus
Aug 23, 2002·Experimental Dermatology·Markus MagerlDesmond J Tobin
Apr 28, 2005·The Journal of Investigative Dermatology·Ying ZhengKurts Stenn
Feb 24, 2006·Nature Reviews. Molecular Cell Biology·Linda G Griffith, Melody A Swartz
May 6, 2008·Tissue Engineering. Part B, Reviews·Jungwoo LeeNicholas A Kotov
Oct 22, 2008·Acta Biomaterialia·Stephen F BadylakThomas W Gilbert
Jan 1, 2007·Organogenesis·K StennK Washenik
Sep 19, 2009·Regenerative Medicine·Jizeng QiaoJeff Teumer
Oct 27, 2009·Developmental Biology·Tania Rozario, Douglas W DeSimone
Dec 22, 2009·Journal of Dermatological Science·Chao-Chun Yang, George Cotsarelis
May 12, 2010·Experimental Dermatology·Claire A HigginsColin A B Jahoda
Jul 2, 2010·Annual Review of Genomics and Human Genetics·Yutaka Shimomura, Angela M Christiano
Mar 30, 2011·Journal of Cell Science·Ryan R DriskellFiona M Watt
May 19, 2012·Expert Opinion on Pharmacotherapy·Mariya Miteva, Antonella Tosti
Sep 11, 2012·Seminars in Cell & Developmental Biology·Rachel Sennett, Michael Rendl
Jul 11, 2013·Nature Reviews. Molecular Cell Biology·Fiona M Watt, Wilhelm T S Huck
Sep 11, 2013·Facial Plastic Surgery Clinics of North America·Bryan T MarshallKen Washenik
Oct 15, 2013·Dermatologic Surgery : Official Publication for American Society for Dermatologic Surgery [et Al.]·Yong MiaoZhi-Qi Hu
Oct 23, 2013·Proceedings of the National Academy of Sciences of the United States of America·Claire A HigginsAngela M Christiano
Mar 4, 2014·Journal of Dermatological Science·Sun-Young YoonKyu Han Kim
Jul 6, 2014·Advanced Drug Delivery Reviews·Gabriel BentonJennifer Koblinski
May 6, 2015·Journal of Clinical and Experimental Hepatology·Denver M FaulkStephen F Badylak
Nov 13, 2015·Advanced Drug Delivery Reviews·Achilleas D TheocharisNikos K Karamanos
Dec 15, 2015·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Xiao LuoCraig M Neville
Jul 28, 2016·The FEBS Journal·Rita Gemayel, Emily J Chenette
May 10, 2017·Actas dermo-sifiliográficas·F Jiménez-Acosta, I Ponce-Rodríguez
Aug 5, 2017·Methods in Molecular Biology·Guobao Chen, Yonggang Lv
Dec 7, 2017·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Zurab KakabadzeSanjeev Gupta
Jan 27, 2019·Bioengineering & Translational Medicine·Yu Seon KimAntonios G Mikos

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Citations

Dec 27, 2019·Stem Cells Translational Medicine·Ana Rita Castro, Elsa Logarinho
Feb 6, 2020·Experimental and Therapeutic Medicine·Qiang ZhouHao Cheng
Feb 18, 2021·Signal Transduction and Targeted Therapy·Shuaifei JiXiaobing Fu
Aug 22, 2021·Colloids and Surfaces. B, Biointerfaces·Priya MishraRahul Shukla

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Methods Mentioned

BETA
enzyme-linked immunosorbent assay
electrophoresis

Software Mentioned

PC densitometry
SPSS17
analyst

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