Use of Feedback-Resistant Threonine Dehydratases of Corynebacterium glutamicum To Increase Carbon Flux towards l-Isoleucine.

Applied and Environmental Microbiology
S MorbachL Eggeling

Abstract

The biosynthesis of l-isoleucine proceeds via a highly regulated reaction sequence connected with l-lysine and l-threonine synthesis. Using defined genetic Corynebacterium glutamicum strains characterized by different fluxes through the homoserine dehydrogenase reaction, we analyzed the influence of four different ilvA alleles (encoding threonine dehydratase) in vectors with two different copy numbers on the total flux towards l-isoleucine. For this purpose, 18 different strains were constructed and analyzed. The result was that unlike ilvA in vectors with low copy numbers, ilvA in high-copy-number vectors increased the final l-isoleucine yield by about 20%. An additional 40% increase in l-isoleucine yield was obtained by the use of ilvA alleles encoding feedback-resistant threonine dehydratases. The strain with the highest yield was characterized by three hom(Fbr) copies encoding feedback-resistant homoserine dehydrogenase and ilvA(Fbr) encoding feedback-resistant threonine dehydratase on a multicopy plasmid. It accumulated 96 mM l-isoleucine, without any l-threonine as a by-product. The highest specific productivity was 0.052 g of l-isoleucine per g of biomass per h. This comparative flux analysis of isogenic strains showed t...Continue Reading

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Citations

Jul 17, 2008·Journal of Biomedicine & Biotechnology·Rafael Moreno-SánchezViridiana Olín-Sandoval
Jun 14, 2015·World Journal of Microbiology & Biotechnology·Yanfeng GuoWeiguo Zhang
Apr 27, 2002·Applied and Environmental Microbiology·Eva RadmacherLothar Eggeling
Oct 22, 2009·Applied Microbiology and Biotechnology·Jin Hwan Park, Sang Yup Lee
Jul 1, 2008·Microbial Biotechnology·Sergio Sanchez, Arnold L Demain
Oct 11, 2014·Applied Microbiology and Biotechnology·Michael VogtLothar Eggeling
Jun 1, 2020·Biotechnology and Applied Biochemistry·Yanchao ZhangXiaoyuan Wang
Jun 3, 2010·Biotechnology Journal·Jin Hwan Park, Sang Yup Lee
Jan 22, 2019·Applied Microbiology and Biotechnology·Xiaoyuan Wang
Oct 13, 2018·Biotechnology and Applied Biochemistry·Wenjian MaXiaoyuan Wang
Jul 19, 2008·Biotechnology and Bioengineering·A MarxL Eggeling
Dec 1, 1996·Applied and Environmental Microbiology·S MorbachL Eggeling
Sep 1, 1996·Applied and Environmental Microbiology·T Hermann, R Kramer
Jul 6, 2010·Mitochondrion·Rafael Moreno-SánchezHans V Westerhoff

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