Use of flow cytometry to identify a Caulobacter 4.5 S RNA temperature-sensitive mutant defective in the cell cycle

Journal of Molecular Biology
E Winzeler, L Shapiro

Abstract

Flow cytometry was used to screen a collection of temperature-sensitive mutants for those blocked at discrete points in the cell cycle with respect to the replicative status of the chromosome. At the non-permissive temperature, one such mutant, LS439, could not initiate new rounds of DNA replication and arrested primarily as cells with two completed chromosomes Extended incubation at the restrictive temperature resulted in filament formation. Following the shift to the restrictive temperature protein synthesis continued, but at a reduced rate. A 0.2 kb fragment of DNA located immediately upstream of the Caulobacter homolog of the Escherichia coli dnaX gene was able to completely rescue the temperature-sensitive phenotype of LS439. The 0.2 kb fragment contained a homolog of the bacterial gene encoding 4.5 S RNA. The original point mutation is predicted to disrupt the stem structure in the 4.5 S RNA thus providing a rationale for the genetic basis of the LS439 phenotype.

Citations

Oct 2, 1998·The EMBO Journal·U Jenal, T Fuchs
Sep 5, 2001·The EMBO Journal·R B JensenL Shapiro
May 12, 2009·Journal of Bacteriology·Lin Cheng, Kenneth C Keiler
Jul 16, 2008·Journal of Bacteriology·Djenann Saint-DicLyn Sue Kahng
Jan 4, 2003·Journal of Bacteriology·Kenneth C Keiler, Lucy Shapiro
Mar 20, 2003·Journal of Bacteriology·Ying Du, Cindy Grove Arvidson
Feb 21, 1998·Proceedings of the National Academy of Sciences of the United States of America·K C QuonG T Marczynski
Apr 5, 2001·Proceedings of the National Academy of Sciences of the United States of America·B GrünenfelderU Jenal
Mar 29, 2001·Proceedings of the National Academy of Sciences of the United States of America·C JacobsL Shapiro
Sep 15, 1999·Proceedings of the National Academy of Sciences of the United States of America·R B Jensen, L Shapiro
May 5, 2004·Proceedings of the National Academy of Sciences of the United States of America·Kathleen R RyanLucy Shapiro
Aug 27, 2016·Nature Microbiology·Emeline LawaréeJean-Yves Matroule
Jun 28, 2005·Molecular Microbiology·Sue-Jean HongKenneth C Keiler
Apr 24, 2007·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Arnold J Bendich
Mar 21, 2008·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·Sangram BaghDavid R McMillen
Jun 30, 1998·APMIS. Supplementum·J Olweus
Apr 19, 2012·Preparative Biochemistry & Biotechnology·Jirka GrosseDaniela Grimm
Sep 5, 2008·Current Protocols in Cytometry·J C Wood
Sep 5, 2008·Current Protocols in Cytometry·Lori A KruegerJohannes Hampl
May 4, 2002·Annual Review of Biophysics and Biomolecular Structure·Larry A SklarEric R Prossnitz
Feb 27, 2003·Molecular Microbiology·Christine JacobsMichael T Laub
Nov 18, 2003·Transfusion Medicine·S Bashir, R Cardigan
May 26, 2001·The American Journal of Gastroenterology·C T BothM D Reis
Sep 5, 2008·Current Protocols in Cytometry·Hazel M DaveyArseny S Kaprelyants
Apr 3, 2013·Current Protocols in Cytometry·Simon JohnsonDavid Coder
Jul 1, 2017·Advanced Biosystems·Suwan N Jayasinghe
Jun 1, 1997·Journal of Bacteriology·E Winzeler, L Shapiro

❮ Previous
Next ❯

Related Concepts

Related Feeds

BioHub - Researcher Network

The Chan-Zuckerberg Biohub aims to support the fundamental research and develop the technologies that will enable physicians to cure, prevent, or manage all diseases in our childrens' lifetimes. The CZ Biohub brings together researchers from UC Berkeley, Stanford, and UCSF. Find the latest research from the CZ Biohub researcher network here.