Use of fluorescent antibody in studies of immunity to cholera in infant mice.

Infection and Immunity
M N GuentzelL J Berry

Abstract

Infant mice 8 days of age were infected orally with virulent, motile, classical or El Tor strains of Vibrio cholerae and with nonmotile mutants of low virulence derived from the same strains. At intervals of 8 and 12 h postinfection, frozen thin sections of the ileum were prepared, stained with fluorescein isothiocyanate-labeled rabbit anti-vibrio antibody, and examined with the fluorescence microscope. The motile organisms were present in larger numbers, especially at 12h, and had penetrated the intervillous spaces and crypts of Lieberkuhn more completely than nonmotile vibrios. Dilution counts were made on various regions of the intestines of infant mice challenged orally 12 h previously with either motile or nonmotile strains of V. cholerae. Greater numbers of organisms were found, especially in the upper intestinal regions, when motile organisms were used. Low numbers of vibrios, limited mostly to the lumen, were seen in the ileum of infant mice infected with motile organisms when the infants were the offspring of mothers that had been immunized with crude flagellar vaccine or a vesicular preparation derived from the vibrio cell surface. The distribution of vibrios in this case was similar to that found in infected infants ...Continue Reading

References

Feb 1, 1977·Infection and Immunity·E R EubanksL J Berry
May 1, 1975·Infection and Immunity·M N Guentzel, L J Berry
Aug 1, 1975·The Journal of Infectious Diseases·J E BellamyD Rowley
May 1, 1973·Infection and Immunity·G D SchrankW F Verwey
Dec 1, 1964·Analytical Biochemistry·R M MCKINNEYG W PEARCE

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Citations

Nov 1, 1977·CRC Critical Reviews in Toxicology·L J Berry
Sep 24, 2015·Antimicrobial Agents and Chemotherapy·Jeffrey H WitheyHemanta Koley
Aug 6, 2008·Infection and Immunity·Stefan SchildAndrew Camilli
Sep 30, 2016·Critical Reviews in Biotechnology·Brahma N SinghKhabat Vahabi
Aug 2, 2020·The Journal of Pharmacy and Pharmacology·Francisca B M SousaJand V R Medeiros
Feb 1, 1977·Infection and Immunity·E R EubanksL J Berry
Jul 1, 1979·Infection and Immunity·R J YanceyL J Berry
Apr 1, 1979·Infection and Immunity·V S BaselskiC D Parker
Sep 1, 1981·Infection and Immunity·L H FieldL J Berry

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