Use of fluorescent in-situ hybridisation in salivary gland cytology: A powerful diagnostic tool

Cytopathology : Official Journal of the British Society for Clinical Cytology
S M EvrardM Courtade-Saidi

Abstract

Salivary gland cytology is challenging because it includes a diversity of lesions and a wide spectra of tumours. Recently, it has been reported that many types of salivary gland tumours have specific molecular diagnostic signatures that could be identified by fluorescent in-situ hybridisation (FISH). The aim of the present study was to demonstrate the feasibility and efficiency of FISH on routine cytological salivary gland smears. FISH was conducted on 37 cytological salivary gland smears from 34 patients. According to the cytological diagnosis suspected, MECT1/MAML2 gene fusion and rearrangements of PLAG1, MYB, or ETV6 were analysed. The presence and percentages of cells that had gene rearrangements were evaluated. Results were compared with the histological surgical samples, available from 26 patients. The PLAG1 rearrangement was observed in 12/20 (60%) cases of pleomorphic adenoma. MECT1/MAML2 gene fusion was observed in 1:2 mucoepidermoid carcinomas but was not observed in five other tumours (two pleomorphic adenomas, one Warthin's tumour, one mammary analogue secretory carcinoma [MASC] and one cystic tumour). MYB rearrangement was observed in 4/4 adenoid cystic carcinomas. ETV6-gene splitting identified one MASC. Overall, ...Continue Reading

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Aug 30, 2018·Cancer Cytopathology·Vickie Y Jo, Jeffrey F Krane
Sep 22, 2018·European Archives of Oto-rhino-laryngology : Official Journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : Affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery·Peter ZbärenAlfio Ferlito
Aug 31, 2020·Laryngoscope Investigative Otolaryngology·Katri AroTimo Atula
May 26, 2021·Cytopathology : Official Journal of the British Society for Clinical Cytology·Yonca KanberManon Auger

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