Use of FTA Cards To Transport Throat Swabs and Oral Fluid Samples for Molecular Detection and Genotyping of Measles and Rubella Viruses

Journal of Clinical Microbiology
Bettina BankampJames L Goodson

Abstract

The genetic characterization of measles viruses is an important tool for measles surveillance. Reverse cold chain requirements for the transportation of samples to reference laboratories are challenging in resource-limited settings. FTA cards facilitate the transport of virologic samples at ambient temperature as noninfectious material; however, the utility of FTA cards for the detection and genotyping of measles virus from clinical samples has not been evaluated. Throat swabs (TS) and oral fluid (OF) samples were collected from suspected measles cases in the Democratic Republic of the Congo. Virus detection (reverse transcription-quantitative real-time PCR [RT-qPCR]) and genotyping (endpoint RT-PCR) were compared for samples from 238 suspected cases; these samples were either transported using the reverse cold chain or at ambient temperature on FTA cards. Virus detection showed excellent positive agreement for OF samples compared to TS (95.3%; confidence interval [CI], 91.6 to 97.4), in contrast to 79.4% (CI, 73.5 to 84.3) for TS on FTA, and 85.5% (CI, 80.2 to 89.6) for OF on FTA compared to OF samples. Genotyping results obtained for a subset of samples indicated that 77.3% of all TS and 71.0% of OF samples would produce geno...Continue Reading

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Citations

Apr 8, 2021·Clinical Chemistry and Laboratory Medicine : CCLM·Gernot KriegshäuserPeter Panhofer
Aug 28, 2021·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·Marilou S PagonendjiJudith M Hübschen
Nov 26, 2021·ACS Biomaterials Science & Engineering·Luciana d'AmoneFiorenzo G Omenetto

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