PMID: 8958448Sep 1, 1996Paper

Use of fumonisin B1 to test the intercellular transfer of sphingosine

Cellular Signalling
S Inooka, T Toyokuni

Abstract

Sphingosine, a core unit of sphingolipids, has been shown to mediate intracellular signaling events. It is conceivable that sphingosine could act as an intracellular messenger as well as an intracellular modulator, if sphingosine were to be transferred intercellularly. Murine interleukin-2 (IL-2) dependent T-lymphocyte CTLL cells, murine fibroblasts Swiss 3R3 cells, and murine fibroblast BALB/C A31 cells metabolize exogenously added sphingosine to ceramide. Fumonisin B2, a mycotoxin produced by Fusarium moniliforme, blocks the conversion of sphingosine to ceramide. In the study described here, CTLL cells exhibiting the conversion of sphingosine to ceramide were used as recipient cells, whereas fumonisin B1-treated cells (A31 cells, Swiss 3T3 cells and CTLL cells), in which the conversion was blocked were used as donor cells. To demonstrate intercellular transfer of sphingosine through the conversion pathway of sphingosine to ceramide, fumonisin B1-treated donor cells incorporating radioactive sphingosine were co-incubated with CTLL cells and the ceramide response was examined. These experiments demonstrated that it is possible to prove the intercellular transfer of sphingosine by using different activities in the cellular conve...Continue Reading

References

Aug 31, 1992·Biochemical and Biophysical Research Communications·P P Van VeldhovenR M Bell
Dec 1, 1987·Experimental Cell Research·E KamJ D Pitts
Aug 8, 1989·Biochimica Et Biophysica Acta·B J AlA W Schram
Mar 19, 1993·Science·L M ObeidY A Hannun

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Biochemical and Biophysical Research Communications
S Inooka, T Toyokuni
Zhonghua yu fang yi xue za zhi [Chinese journal of preventive medicine]
Xiu-mei LiuX Li
FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology
S Spiegel, A H Merrill
© 2021 Meta ULC. All rights reserved