PMID: 9182868Jan 1, 1997Paper

Use of gas chromatographic-mass spectrometric techniques in studies of androst-16-ene and androgen biosynthesis in human testis; cytosolic specific binding of 5alpha-androst-16-en-3-one

The Journal of Steroid Biochemistry and Molecular Biology
T K KwanD B Gower

Abstract

Homogenates of histologically normal human testis from three men were incubated separately with pregnenolone, 16-dehydropregnenolone, 5alpha-pregnane-3,20-dione, 3beta-hydroxy-5alpha-pregnan-20-one and androsta-5,16-dien-3beta-ol (androstadienol) in the presence of NADPH in a study of androst-16-ene and androgen biosynthesis. After the addition of internal standards and initial extraction and purification, metabolites were identified using gas chromatography-mass spectrometry (GC-MS) and monitoring selectively for three principal ions in each case at the appropriate GC retention time. Quantification was achieved by comparison with calibration lines for authentic steroids, together with the appropriate internal standards, prepared by monitoring three ion fragments for each analyte. In all experiments, androstadienol was found to be the major androst-16-ene metabolite of pregnenolone (seven times the control, i.e. endogenous, quantity; 19.8 +/- 3 ng/100 mg homogenate protein, mean +/- SEM, n = 9). Pregnenolone was also converted to androsta-4,16-dien-3-one (androstadienone) with three times the endogenous quantity (44 +/- 10 ng/100 mg homogenate protein, mean +/- SEM, n = 9) being formed. The formation of testosterone occurred on...Continue Reading

References

Jan 1, 1977·Psychoneuroendocrinology·J J CowleyB W Brooksbank
Aug 1, 1976·Journal of Steroid Biochemistry·G KaufmannK Schubert
May 1, 1992·Biochemical Society Transactions·M A KraevskayaD B Gower
Nov 1, 1992·The Journal of Steroid Biochemistry and Molecular Biology·T K KwanD B Gower
Nov 1, 1992·Biochemical Society Transactions·M A KraevskayaD B Gower
Oct 1, 1991·The Journal of Steroid Biochemistry and Molecular Biology·J J Cowley, B W Brooksbank
Jan 1, 1991·The Journal of Steroid Biochemistry and Molecular Biology·A G Smals, J J Weusten
May 1, 1989·Journal of Steroid Biochemistry·J J WeustenT Benraad
Apr 1, 1986·Biological Psychology·D Benton, V Wastell
Apr 11, 1972·Biochemistry·A RuokonenR Vihko
Jul 1, 1972·The Biochemical Journal·P J Brophy, B D Gower
Apr 1, 1994·Baillière's Clinical Endocrinology and Metabolism·V A Randall

❮ Previous
Next ❯

Citations

May 18, 2005·The Journal of Steroid Biochemistry and Molecular Biology·P A SinclairE J Squires
Jun 16, 2005·The Journal of Steroid Biochemistry and Molecular Biology·P A SinclairR Renaud
Oct 27, 2004·Physiology & Behavior·Bettina M Pause
Oct 4, 2002·The Journal of Steroid Biochemistry and Molecular Biology·A K D PertiwiD B Gower
May 31, 2008·The Journal of Steroid Biochemistry and Molecular Biology·Galia ZamaratskaiaOystein Andresen
Jun 22, 2002·Journal of Chromatography. a·Patrik Appelblad, Knut Irgum

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.