Use of Gen-Probe AccuProbe Group B streptococcus test to detect group B streptococci in broth cultures of vaginal-anorectal specimens from pregnant women: comparison with traditional culture method.

Journal of Clinical Microbiology
P P BourbeauM Figdore

Abstract

Detection of vaginal-anorectal colonization with group B streptococci (GBS) is critical to the prevention of neonatal GBS disease. The recommended method for the detection of GBS is culture of the distal vagina and anorectum in a selective broth medium followed by subculture to solid media and identification of GBS on the solid media. The purpose of this study was to compare this standard culture method with the detection of GBS directly from an enrichment broth by utilizing the Gen-Probe AccuProbe Group B Streptococcus Culture Identification Test (GPGB). A total of 502 specimens were tested in this study. Both culture and the GPGB detected 90 of 95 positive specimens (sensitivity, 94.7%). There were two false-positive GPGB results (specificity, 99.5%). An analysis of 100 consecutive specimens was performed to compare the costs associated with the use of a primary tryptic soy agar plate with 5% sheep blood (BAP) and a 3-ml tube of Todd-Hewitt broth supplemented with 10 micrograms of nalidixic acid per ml and 15 micrograms of colistin per ml (LIM broth) with subculture to another BAP and the costs associated with the GPGB. Our estimated costs were $3.68 for a negative culture including 7.0 min of labor, $5.41 for a positive cult...Continue Reading

References

Sep 1, 1983·Journal of Clinical Microbiology·D E JonesD V Lim

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Citations

Jul 5, 2013·Clinical and Vaccine Immunology : CVI·Hidehito MatsuiHideaki Hanaki
May 14, 2005·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·H Réglier-PoupetC Poyart
May 30, 2014·The Journal of Microbiology·Yun Gyu SongKwang-Ho Rhee
Apr 21, 2009·Analytical and Bioanalytical Chemistry·Jan Weile, Cornelius Knabbe
Mar 4, 2003·Obstetrical & Gynecological Survey·Julie S Platt, William F O'Brien
Aug 7, 2004·Journal of Clinical Microbiology·François J PicardMichel G Bergeron
Jan 10, 2012·Analytical Chemistry·Vladimir GubalaDavid E Williams

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