Use of Gen-Probe and Bactec for rapid isolation and identification of mycobacteria. Correlation of probe results with growth index

American Journal of Clinical Pathology
B A BodyM Chery

Abstract

Gen-Probe culture confirmation tests (Gen-Probe, San Diego, CA) for Mycobacterium tuberculosis complex and Mycobacterium avium complex were performed on 276 mycobacterial isolates. All 138 M. tuberculosis complex isolates and 79 of 80 M. avium complex isolates were identified correctly. No falsely positive test results were obtained; 58 nontuberculous mycobacteria other than M. avium complex were negative by Gen-Probe. In a second phase of testing, Gen-Probe tests were performed using concentrates from 101 patient Bactec 12B cultures. Positive results by Gen-Probe tests were correlated with the growth index (GI) reading on the day of processing as well as the accumulated GI readings. For those 51 with high (greater than or equal to 999) final GIs, 40/40 (100%) M. tuberculosis complex isolates and 9/11 M. avium complex isolates were positive by Gen-Probe, and six other mycobacteria were negative. Of the 25 with moderate final readings (400 less than or equal to GI less than 999), 12/17 M. tuberculosis complex isolates and 1/1 M. avium complex isolates were correctly identified by Gen-Probe; seven other mycobacteria were negative. Of 25 with low readings (GI less than 400), 8/24 M. tuberculosis isolates were correctly identified ...Continue Reading

Citations

Apr 1, 1996·Critical Reviews in Clinical Laboratory Sciences·C D HeroldD A Herold
Mar 1, 1993·The Journal of Urology·J R FishmanR W deVere White
Jan 1, 1992·Journal of Clinical Microbiology·D V CousinsB L Gow
Oct 1, 1991·Journal of Clinical Microbiology·T E Davis, D D Fuller
Aug 15, 1998·Journal of Clinical Microbiology·E TortoliM T Simonetti
Feb 1, 1995·Molecular and Cellular Probes·T B Britschgi, G A Cangelosi
Apr 1, 1993·Seminars in Roentgenology·W T Miller, W T Miller

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