Use of glutamine and low density lipoproteins isolated from egg yolk to improve buck semen freezing

Reproduction in Domestic Animals = Zuchthygiene
M Z Ali Al AhmadF Fieni

Abstract

To improve the results obtained with a reference cryopreservation extender (control extender: Triladyl + 20% (v/v) egg yolk + 6.4% (v/v) glycerol) for freezing caprine semen, glutamine was added to 18 split ejaculates at concentrations of 0, 20, 40, 80 and 120 mM (experiment 1). In experiment 2, glutamine was added to 18 split ejaculates at concentrations of 20, 25, 30, 35 and 40 mM. In the third experiment, the egg yolk was replaced with the low-density lipoprotein (LDL) fraction of egg yolk. The quality of frozen then thawed spermatozoa in each extender was compared using computer-assisted semen analysis. In experiment 1, glutamine at concentrations of 20 mm and 40 mm significantly improved sperm motility compared with the control extender. However, at 120 mM, a significant decrease in motility and velocity was observed. In experiment 2, motility, curvilinear velocity and amplitude of lateral head displacement (ALH) were improved in glutamine at 25 mM compared with the control. In experiment 3, 8% LDL and 25 mM glutamine significantly improved sperm motility, straight line velocity and ALH. In the fourth experiment, the quality of the previously defined freezing extender (Triladyl + 8% (v/v) LDL + 25 mM glutamine + 6.4% (v/v)...Continue Reading

References

Apr 1, 1975·Australian Journal of Biological Sciences·P F Watson, I C Martin
Jan 1, 1992·Reproduction, Fertility, and Development·L G Sánchez-PartidaB P Setchell
Feb 1, 1989·Zentralblatt für Veterinärmedizin. Reihe A·E KoskinenH Soini
Dec 22, 1988·Biochimica Et Biophysica Acta·T AnchordoguyJ H Crowe
Jun 1, 1988·Cryobiology·J F Carpenter, J H Crowe
Dec 1, 1974·Journal of Animal Science·M M Pace, E F Graham
Dec 12, 2003·Fertility and Sterility·Aleksander GiwercmanMarcello Spano
Dec 16, 2004·Reproduction in Domestic Animals = Zuchthygiene·F Marco-JiménezM Rodriguez
Mar 31, 2005·Reproduction : the Official Journal of the Society for the Study of Fertility·Lamia AmiratJean Luc Courtens
Dec 6, 2005·Reproduction in Domestic Animals = Zuchthygiene·L Fraser, J Strzezek
Apr 1, 1996·Theriogenology·A TrimecheD Tainturier

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Citations

Jul 11, 2012·Cell and Tissue Research·Maria Manuela FrancouJoaquín De Juan
Jun 8, 2012·Animal Reproduction Science·Wei ZhangXu Zhou
Jan 3, 2009·Animal Reproduction Science·E de MercadoJ Roca
Jul 6, 2014·Zygote : the Biology of Gametes and Early Embryos·Sirirak BuarpungMongkol Techakumphu
Oct 16, 2012·Theriogenology·J D BensonJ K Critser
Jul 13, 2020·Reproduction in Domestic Animals = Zuchthygiene·Parisa NazariJalal Rostamzadeh
Jan 30, 2020·Frontiers in Cell and Developmental Biology·Muhammet Rasit UgurErdogan Memili

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