Use of green fluorescent protein to detect expression of nif genes of Azoarcus sp. BH72, a grass-associated diazotroph, on rice roots

Molecular Plant-microbe Interactions : MPMI
T EgenerB Reinhold-Hurek

Abstract

A gfp (green fluorescent protein) cassette for transcriptional fusions has been developed to study gene expression in Azoarcus sp. BH72 in association with plant roots. The bacteria expressed nitrogenase genes (nifHDK) in the rhizosphere, on root tips, and in epidermal cells of rice seedlings. Green fluorescent protein fusions also visualized promoter activity of single cells in soil.

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Citations

Dec 4, 2003·Journal of Biotechnology·Thomas Hurek, Barbara Reinhold-Hurek
Feb 5, 1999·Gene·A K Dunn, J Handelsman
May 20, 1998·Trends in Microbiology·B Reinhold-Hurek, T Hurek
Jun 13, 2012·Integrative Biology : Quantitative Biosciences From Nano to Macro·Yongfan MenHongwei Guo
Oct 24, 1998·Molecular Microbiology·J DörrB Reinhold-Hurek
Jan 18, 2011·Journal of Experimental Botany·Tatiana KraiserRodrigo A Gutiérrez
Jan 13, 2012·Molecular Plant-microbe Interactions : MPMI·Anna BuschartBarbara Reinhold-Hurek
Aug 19, 2011·Molecular Plant-microbe Interactions : MPMI·Andrea KrauseBarbara Reinhold-Hurek
Mar 15, 2006·Molecular Plant-microbe Interactions : MPMI·Barbara Reinhold-HurekThomas Hurek
Apr 16, 2002·Molecular Plant-microbe Interactions : MPMI·Thomas HurekYves Piché
Feb 2, 2000·Applied and Environmental Microbiology·M K Chelius, E W Triplett
Oct 27, 2001·Applied and Environmental Microbiology·A ElbeltagyK Minamisawa
Jan 1, 2008·Microbes and Environments·Junko Terakado-TonookaShinsuke Fujihara
Mar 29, 2001·Proceedings of the National Academy of Sciences of the United States of America·R M BringhurstD J Gage
Oct 31, 2002·Critical Reviews in Biotechnology·Moul Dey, Swapan K Datta
Oct 20, 2005·Environmental Microbiology·Michael RothballerAnton Hartmann
Jun 26, 2003·Environmental Microbiology·Jonathan P ZehrGrieg F Steward
Dec 23, 2011·Molecular Microbiology·Abhijit SarkarBarbara Reinhold-Hurek
Mar 14, 2008·FEMS Microbiology Ecology·Pilar Prieto, Jesús Mercado-Blanco
Nov 20, 2016·Proceedings of the National Academy of Sciences of the United States of America·Rafal ZgadzajSimona Radutoiu
Aug 4, 2019·World Journal of Microbiology & Biotechnology·Romeu FranciscoPaula V Morais
Apr 4, 2021·Life·Valentina LevakAnna Coll
Jul 13, 2021·Frontiers in Microbiology·Timothy L HaskettPhilip S Poole

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