PMID: 9647829Jul 2, 1998Paper

Use of green fluorescent protein to tag and investigate gene expression in marine bacteria

Applied and Environmental Microbiology
S StrettonA E Goodman

Abstract

Two broad-host-range vectors previously constructed for use in soil bacteria (A. G. Matthysse, S. Stretton, C. Dandie, N. C. McClure, and A. E. Goodman, FEMS Microbiol. Lett. 145:87-94, 1996) were assessed by epifluorescence microscopy for use in tagging three marine bacterial species. Expression of gfp could be visualized in Vibrio sp. strain S141 cells at uniform levels of intensity from either the lac or the npt-2 promoter, whereas expression of gfp could be visualized in Psychrobacter sp. strain SW5H cells at various levels of intensity only from the npt-2 promoter. Green fluorescent protein (GFP) fluorescence was not detected in the third species, Pseudoalteromonas sp. strain S91, when the gfp gene was expressed from either promoter. A new mini-Tn10-kan-gfp transposon was constructed to investigate further the possibilities of fluorescence tagging of marine bacteria. Insertion of mini-Tn10-kan-gfp generated random stable mutants at high frequencies with all three marine species. With this transposon, strongly and weakly expressed S91 promoters were isolated. Visualization of GFP by epifluorescence microscopy was markedly reduced when S91 (mini-Tn10-kan-gfp) cells were grown in rich medium compared to that when cells were g...Continue Reading

References

Nov 1, 1982·Journal of Bacteriology·F E Nano, S Kaplan
Feb 23, 1995·Nature·R HeimR Y Tsien
Feb 11, 1994·Science·M ChalfieD C Prasher
Sep 1, 1995·Molecular Microbiology·L KremerC Locht
Nov 15, 1996·FEMS Microbiology Letters·A G MatthysseA E Goodman
Nov 15, 1997·Applied and Environmental Microbiology·T Tolker-NielsenS Molin
Nov 15, 1997·Applied and Environmental Microbiology·G V BloembergR Kolter
Jan 1, 1990·Applied and Environmental Microbiology·N H AlbertsonS Kjelleberg
Mar 1, 1993·Applied and Environmental Microbiology·M L AnglesA E Goodman

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Citations

Dec 8, 2005·Applied and Environmental Microbiology·Richard A LongFarooq Azam
Apr 27, 2000·Journal of Bacteriology·P Watnick, R Kolter
Jan 5, 2000·Applied and Environmental Microbiology·M C GeoffroyA Mercenier
Sep 27, 2008·Extremophiles : Life Under Extreme Conditions·Corien BakermansMichael F Thomashow
Aug 29, 2013·Molecular Ecology·Mary T H D NguyenTorsten Thomas
Jun 7, 2015·BMC Microbiology·Sarah K GilesMelissa H Brown
Sep 29, 2011·Environmental Microbiology·Jenna M YoungGemma Reguera
Sep 3, 1999·Applied and Environmental Microbiology·C SternbergS Molin
Mar 7, 2021·Microorganisms·Breanne M HeadAnn Karen C Brassinga
Dec 28, 1999·Methods : a Companion to Methods in Enzymology·W Margolin

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