PMID: 8955397Dec 1, 1996Paper

Use of green fluorescent protein to visualize the early events of symbiosis between Rhizobium meliloti and alfalfa (Medicago sativa)

Journal of Bacteriology
D J GageS R Long

Abstract

A gene encoding a variant of green fluorescent protein (GFP) of Aequorea victoria was put under the control of a promoter which is constitutive in Rhizobium meliloti. The heterologous GFP gene was expressed at high levels during all stages of symbiosis, allowing R. meliloti cells to be visualized as they grew in the rhizosphere, on the root surface, and inside infection threads. In addition, nodules that were infected with bacteria which were synthesizing GFP fluoresced when illuminated with blue light. GFP-tagged bacteria could be seen inside infection threads, providing the opportunity to measure the growth rate and determine the patterns of growth of R. meliloti residing inside its host plant.

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Citations

Sep 24, 1999·Molecular Plant-microbe Interactions : MPMI·E B HeinzW R Streit
Mar 23, 2001·Canadian Journal of Microbiology·K HeinrichP J Murphy
Dec 4, 2003·Molecular Plant-microbe Interactions : MPMI·Wen-Ming ChenJanet I Sprent
May 27, 2005·Molecular Plant-microbe Interactions : MPMI·Alejandra L D'AntuonoViviana C Lepek
Nov 24, 2007·FEMS Microbiology Letters·Robert P RyanDavid N Dowling
Aug 7, 2012·Applied and Environmental Microbiology·M C QuecineA A Pizzirani-Kleiner
May 29, 2012·Journal of Bacteriology·Hai-Yang LuHai-Ping Cheng
Aug 30, 2000·Proceedings of the National Academy of Sciences of the United States of America·S V NyholmM J McFall-Ngai
Jan 5, 2002·Journal of Food Protection·K Takeuchi, J F Frank
Jul 3, 2004·Nature Reviews. Molecular Cell Biology·Giles E D Oldroyd, J Allan Downie
Apr 17, 2008·Archives of Microbiology·Serena CameriniRoberto Defez
Feb 11, 2004·FEMS Microbiology Letters·Hai-Ping Cheng, Shi-Yi Yao
Dec 19, 2008·Plant Cell Reports·Jean-Michel Hily, Zongrang Liu
Oct 2, 2009·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Pedro T LimaElio Sucena
Mar 6, 2019·Archives of Microbiology·Pramod Kumar PandeyRaj Narain Singh Yadav
Nov 4, 2000·Molecular Plant-microbe Interactions : MPMI·A C TimmersG Truchet
Mar 29, 2001·Proceedings of the National Academy of Sciences of the United States of America·R M BringhurstD J Gage
Jul 27, 2005·Molecular Plant-microbe Interactions : MPMI·John Beck JensenT V Bhuvaneswari
Apr 1, 2004·FEMS Microbiology Ecology·Kieran GermaineDavid N Dowling
Dec 7, 2007·Molecular Plant-microbe Interactions : MPMI·Alejandra L D'AntuonoViviana C Lepek
Jan 13, 2010·Molecular Plant-microbe Interactions : MPMI·Chunxia WangBruno W S Sobral
Nov 12, 2014·Molecular Plant-microbe Interactions : MPMI·Barney A GeddesIvan J Oresnik

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