PMID: 11920631Mar 29, 2002Paper

Use of H19 regulatory sequences for targeted gene therapy in cancer

International Journal of Cancer. Journal International Du Cancer
P OhanaA Hochberg

Abstract

We present a tumor gene therapy approach based on the use of regulatory sequences of the H19 gene that are differentially expressed between normal and cancer cells. We constructed expression vectors carrying the gene for the A fragment of diphtheria toxin (DT-A) or herpes simplex virus thymidine kinase (HSV-tk), under the control of a 814 bp 5'-flanking region of the H19 gene. The cell killing activity of these constructs was in accordance with the relative activity of the H19 regulatory sequences in the transfected cells. We evaluated the therapeutic potential of the gene expression constructs driven by H19 regulatory sequences in an animal model of bladder cancer induced by subcutaneous injection of syngeneic bladder tumor cell lines. Intratumoral injection of these constructs caused a significant suppression of subcutaneous tumor growth, with no obvious toxicity toward the host.

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Citations

Jan 30, 2013·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Man-Tang QiuLin Xu
Sep 29, 2005·Journal of Clinical Pathology·Y FelligA Hochberg
Jun 21, 2011·PloS One·Günther HasenpuschCarsten Rudolph
Nov 10, 2011·Toxins·Assaf Shapira, Itai Benhar
Oct 19, 2010·Drug Development and Industrial Pharmacy·Soo Hyun JangHwa Jeong Lee
Nov 6, 2010·Journal of Oncology·V ScaiewiczA Czerniak
Jan 12, 2005·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·Nadarajah VigneswaranWolfgang Zacharias
Sep 21, 2004·Molecular Carcinogenesis·Ilana ArielAbraham Hochberg
May 3, 2003·Molecular Therapy : the Journal of the American Society of Gene Therapy·B AyeshA Hochberg
Jan 24, 2017·Cancer Letters·Ying TangTao Liu
Apr 4, 2018·Cancers·Mizuho Sato-DahlmanMasato Yamamoto
Aug 26, 2011·Intervirology·Yantao WuSongshu Meng
Mar 11, 2015·International Journal of Gynecological Cancer : Official Journal of the International Gynecological Cancer Society·Chengcheng RenGuang-mei Zhang
Aug 16, 2019·Expert Opinion on Investigational Drugs·Jennifer TseVitaly Margulis
Nov 5, 2019·Frontiers in Microbiology·Fatemeh ShafieeAli Jahanian-Najafabadi
Nov 3, 2004·The Journal of Gene Medicine·Patricia OhanaAbraham Hochberg
Nov 8, 2003·World Journal of Gastroenterology : WJG·Chao Zhang, Zhan-Kui Liu
Nov 23, 2020·Cancer Letters·Yun Wei Shermane LimBoon-Cher Goh

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