Use of human and rat keratinocyte cultures to assess skin irritation potential

Toxicology in Vitro : an International Journal Published in Association with BIBRA
J N LawrenceD J Benford

Abstract

An in vitro cell culture approach was evaluated for its ability to provide data pertinent to the assessment of skin irritation potential. The hypothesis of this approach is that a direct toxic insult to the epidermal keratinocyte in vivo may lead to release of inflammatory mediators, which are responsible for initiation of a local primary skin irritant reaction. This paper presents data on the cytotoxic potential of a number of structurally unrelated chemicals (chloroform, 2-methoxyethanol, 2-butoxyethylacetate, toluene, 1-butanol, acetaldehyde, n-hexane, sodium dodecyl sulfate, benzalkonium chloride, silver nitrate, dibutyltin dichloride and tributyltin chloride). Cytotoxicity (neutral red uptake and intracellular acid phosphatase activity) of a number of structurally unrelated chemicals, representative of a wide range of skin irritation potential, was evaluated in cultures of rat and human epidermal keratinocytes. The sensitivity of human and rat keratinocytes to the test chemicals was very similar, irrespective of the endpoint of cytotoxicity. The neutral red uptake assay appeared more generally applicable to the diverse range of chemical structures represented in this study, since not all test chemicals elicited an early in...Continue Reading

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Jul 1, 1993·Toxicology in Vitro : an International Journal Published in Association with BIBRA·F M DicksonD J Benford

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Citations

Jul 25, 2000·Toxicology in Vitro : an International Journal Published in Association with BIBRA·J K LeeP Y Kim
Jun 1, 1997·Toxicology in Vitro : an International Journal Published in Association with BIBRA·J N Lawrence
Oct 1, 1997·Toxicology in Vitro : an International Journal Published in Association with BIBRA·J N LawrenceD J Benford
Nov 19, 2013·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Young-Su YangKyuhong Lee
Jun 29, 2005·International Journal of Toxicology·UNKNOWN Cosmetic Ingredient Review Expert Panel
Sep 1, 1999·Alternatives to Laboratory Animals : ATLA·J van de SandtM Fartasch

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