Use of human hepatocytes to investigate blood coagulation factor

Methods in Molecular Biology
Christine Biron-AndréaniPatrick Maurel

Abstract

Coagulation is the complex process by which activation of plasmatic haemostasis proteins ends up with the generation of fibrin. Most of the plasma coagulation proteins are synthesized in hepatocytes. The aim of this chapter is to describe experimental procedures allowing to measure the secretion by primary human hepatocytes and functional activity (including production of fibrillar material from extracellular medium) of haemostasis proteins including factors II, V, VII, VIII, PIVKA-II (protein induced by vitK 1 absence or antagonist II), antithrombin and protein S. In addition, we show how treatments of hepatocyte cultures with vitamin K and/or warfarin affect the secretion of haemostasis proteins. The results demonstrate that primary cultures of human hepatocytes constitute an invaluable model for investigating haemostasis protein expression and activity and therapeutic strategies targeting these proteins.

Citations

Oct 25, 2011·PLoS Pathogens·Noëlla ArnaudEliane F Meurs
Feb 24, 2015·Annals of Hematology·Milla Elvi Linnea KuusistoOuti Kuittinen
Jul 6, 2021·Current Opinion in Hematology·Rinku Majumder, Tina Nguyen

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