Use of hybrid chitosan membranes and N1E-115 cells for promoting nerve regeneration in an axonotmesis rat model

Biomaterials
Sandra AmadoS Geuna

Abstract

Many studies have been dedicated to the development of scaffolds for improving post-traumatic nerve regeneration. The goal of this study was to develop and test hybrid chitosan membranes to use in peripheral nerve reconstruction, either alone or enriched with N1E-115 neural cells. Hybrid chitosan membranes were tested in vitro, to assess their ability in supporting N1E-115 cell survival and differentiation, and in vivo to assess biocompatibility as well as to evaluate their effects on nerve fiber regeneration and functional recovery after a standardized rat sciatic nerve crush injury. Functional recovery was evaluated using the sciatic functional index (SFI), the static sciatic index (SSI), the extensor postural thrust (EPT), the withdrawal reflex latency (WRL) and ankle kinematics. Nerve fiber regeneration was assessed by quantitative stereological analysis and electron microscopy. All chitosan membranes showed good biocompatibility and proved to be a suitable substrate for plating the N1E-115 cellular system. By contrast, in vivo nerve regeneration assessment after crush injury showed that the freeze-dried chitosan type III, without N1E-115 cell addition, was the only type of membrane that significantly improved posttraumatic...Continue Reading

References

Feb 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·Y KimhiU Z Littauer
Jan 1, 1990·Biomaterials, Artificial Cells, and Artificial Organs·T Chandy, C P Sharma
Mar 1, 1985·Plastic and Reconstructive Surgery·S E MackinnonD A Hunter
Jan 1, 1986·Physiology & Behavior·K A Clarke, A J Parker
Sep 1, 1994·Journal of Reconstructive Microsurgery·G LundborgQ Zhao
Nov 4, 1998·Anatomy and Embryology·C Schmitz
Feb 15, 2001·Experimental Neurology·R Koka, T A Hadlock
Oct 13, 2001·Journal of Reconstructive Microsurgery·G M BeerV E Meyer
Oct 29, 2002·Muscle & Nerve·Artur S P VarejãoDavid A Winter
Oct 7, 2003·Annual Review of Biomedical Engineering·Christine E Schmidt, Jennie Baier Leach
Jan 30, 2004·Microsurgery·Stefano GeunaAntonio de Castro Rodrigues
Apr 10, 2004·Neurological Research·Artur S VarejãoJosé Bulas-Cruz
Jul 28, 2004·Biomaterials·Yuki ShirosakiMaria Helena Fernandes
Sep 30, 2004·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Paula Rulf MarrecoAngela Maria Moraes
Feb 3, 2005·Journal of Neurotrauma·Artur S P VarejãoMaria G Giacobini-Robecchi
Feb 22, 2005·Biomaterials·Thomas FreierMolly S Shoichet
Oct 14, 2006·Neurosurgery·Burkhard SchlosshauerNektarios Sinis
Mar 21, 2007·Journal of Biomedical Materials Research. Part a·N IshikawaC Ide
Jun 5, 2007·Biomaterials·Alexis M PietakMichael Sayer
Jun 29, 2007·Microsurgery·S GeunaI Perroteau
Oct 9, 2007·Microsurgery·William C Lineaweaver
Mar 6, 2008·Microscopy Research and Technique·Federica Di ScipioStefano Geuna

❮ Previous
Next ❯

Citations

May 24, 2012·Annals of Biomedical Engineering·Anuradha SubramanianSwaminathan Sethuraman
Jan 27, 2010·Journal of Materials Science. Materials in Medicine·Lei Zheng, Hui-Fei Cui
Jun 5, 2012·Journal of Materials Science. Materials in Medicine·Lei Zheng, Hui-Fei Cui
Jul 8, 2009·Tissue Engineering. Part C, Methods·Xiaoming HeGuoping Chen
May 21, 2010·Journal of Biomaterials Science. Polymer Edition·Xi LiuXingdong Zhang
Nov 27, 2009·Journal of Biomedical Science·Anuradha SubramanianSwaminathan Sethuraman
Mar 8, 2012·Journal of Visualized Experiments : JoVE·Andrew R Bauder, Toby A Ferguson
Feb 18, 2016·Stem Cells International·Ana Rita CaseiroAna Colette Maurício
Aug 24, 2010·Micron : the International Research and Review Journal for Microscopy·Fátima Rosalina Pereira LopesAna Maria Blanco Martinez
Oct 6, 2012·Journal of Tissue Engineering and Regenerative Medicine·Young-Phil KimJung Keun Hyun
Mar 25, 2016·Biointerphases·Christian Fernández-Solis, Andreas Erbe
Feb 4, 2014·Journal of Biomedical Materials Research. Part a·Nuno AlexandreAna Lúcia Luís
Oct 22, 2013·Biomaterials·Haixing XuPeter X Ma
Apr 9, 2011·Annals of Anatomy = Anatomischer Anzeiger : Official Organ of the Anatomische Gesellschaft·Stefania RaimondoStefano Geuna
Mar 8, 2013·Carbohydrate Polymers·Stefan SpirkKarin Stana-Kleinschek
Jan 30, 2015·Journal of Neuroscience Methods·Stefano Geuna
Jul 24, 2014·BioMed Research International·Yuki ShirosakiAna C Mauricio
Nov 26, 2014·PloS One·Tiago PereiraAna Colette Maurício
Jan 13, 2017·Journal of Biomedical Materials Research. Part a·Jorge RibeiroAna Colette Maurício
Feb 19, 2019·Journal of Biomaterials Science. Polymer Edition·Irene CarmagnolaGianluca Ciardelli
Apr 10, 2019·Polymers·Sanjay TiwariPratap Bahadur
Dec 20, 2018·Journal of Cellular Physiology·Bharath Kumar VelmuruganRathinasamy Baskaran
May 27, 2016·Medical Science Monitor : International Medical Journal of Experimental and Clinical Research·Wei YueYong Ji
Nov 6, 2013·Biological & Pharmaceutical Bulletin·Kanji TsuruAkiyoshi Osaka
Feb 14, 2014·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Louise S ConnellJulian R Jones

❮ Previous
Next ❯

Related Concepts

Related Feeds

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis