PMID: 2123859Dec 1, 1990Paper

Use of integrational plasmid excision to identify cellular localization of gene expression during sporulation in Bacillus subtilis

Journal of Bacteriology
N IllingJ Errington

Abstract

Sporulation in Bacillus subtilis is a simple developmental system involving the differentiation of two sister cells, the prespore and the mother cell. Many of the genes that regulate sporulation (spo genes) are thought to be expressed differentially. However, direct demonstration of differential gene expression, by fractionation of prespore and mother cell proteins, is possible only at a relatively late stage of development. H. De Lencastre and P. J. Piggot (J. Gen. Microbiol. 114:377-389, 1979) have described a genetic method for determining the cellular location of the requirement for spo gene expression. Here we describe a similar method based on the use of integrational plasmids that can insertionally inactivate any given spo gene. Loss of the integrated plasmid by homologous recombination leads to the restoration of spo gene function. If this occurs just before sporulation begins, the phenotypes of the progeny of heat-resistant spores should depend on whether the gene is required in the prespore or the mother cell. Thus, we show that for known prespore-specific genes, such as spoIIIG and spoVA, only phenotypically Spo+ progeny that have lost the integrated plasmid are produced. In contrast, for mother-cell-specific genes, ...Continue Reading

References

Oct 1, 1979·Journal of General Microbiology·H de Lencastre, P J Piggot
Mar 1, 1986·Journal of Bacteriology·S RongA L Sonenshein
May 1, 1989·Journal of Bacteriology·M Young, S D Ehrlich
Feb 1, 1989·Genes & Development·C Karmazyn-CampelliP Stragier
Oct 1, 1989·Journal of Bacteriology·A Gholamhoseinian, P J Piggot
Feb 1, 1988·Journal of Bacteriology·S M Cutting, J Mandelstam
Sep 1, 1988·Journal of Bacteriology·V Vagner, S D Ehrlich
May 1, 1961·Journal of Bacteriology·C Anagnostopoulos, J Spizizen

❮ Previous
Next ❯

Citations

Mar 1, 1992·Molecular Microbiology·J Errington, N Illing
Feb 1, 1995·Journal of Bacteriology·N Frandsen, P Stragier
Mar 1, 1995·Microbiological Reviews·W G Haldenwang

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.