Use of isogenic strains indicates CYP9M10 is linked to permethrin resistance in Culex pipiens quinquefasciatus

Insect Molecular Biology
M C HardstoneJ G Scott

Abstract

Previous studies on a strain of Culex pipiens quinquefasciatus from Saudi Arabia indicated permethrin resistance was a result of cytochrome P450 mediated detoxification and kdr. The P450 detoxification was found to be larval specific and associated with a fitness cost in certain environments. The P450 responsible for resistance (and the fitness cost) has not been identified, but recently two candidate P450s (CYP4H34 and CYP9M10) have been found. We measured cytochrome P450 and cytochrome b₅ content as well as the expression levels of CYP4H34 and CYP9M10 in a susceptible (SLAB) and two isogenic strains (isolated by repeated backcrossing and selection) of mosquito (ISOP450 and ISOJPAL) resistant to permethrin. Cytochrome P450 protein levels of the resistant strains were significantly higher (1.5-fold) than SLAB, but were not significantly different from one another. Expression of CYP4H34 in the larvae and adults of the resistant (ISOP450 and ISOJPAL) and susceptible (SLAB) strains were not statistically different. CYP9M10 was found to be significantly over-expressed in larvae of both permethrin-resistant isogenic strains (1800-fold in ISOP450 and 870-fold in ISOJPAL) when compared to SLAB. Partial sequence analysis of CYP9M10 rev...Continue Reading

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Citations

Jan 9, 2013·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Jean-Philippe DavidMark John Ingraham Paine
Oct 25, 2013·International Journal of Biological Sciences·Youhui GongNannan Liu
Jan 22, 2015·International Journal of Molecular Sciences·Xiao LiangKun Yan Zhu
May 20, 2015·Pesticide Biochemistry and Physiology·Nannan LiuTing Li
May 20, 2015·Pesticide Biochemistry and Physiology·Jeffrey G ScottShinji Kasai
Nov 6, 2012·Insect Biochemistry and Molecular Biology·Theresia Estomih NkyaJean-Philippe David
Aug 16, 2016·Pesticide Biochemistry and Physiology·Jianhua ZhangZewen Liu
Oct 30, 2016·Insect Biochemistry and Molecular Biology·Yixi ZhangZewen Liu
Mar 6, 2019·The American Journal of Tropical Medicine and Hygiene·Nguyen Thi Kim LienNguyen Thi Huong Binh
Aug 8, 2019·Scientific Reports·Walter Fabricio Silva MartinsMartin James Donnelly

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