Use of lectin binding characteristics to identify gastrointestinal parasite eggs in faeces

Veterinary Parasitology
I G ColditzR Hosse

Abstract

Identification of the presence and abundance of species is important when choosing therapies and control strategies for internal parasitism of livestock. Here we examine lectin binding characteristics of eggs isolated from sheep faeces as a means for identifying the parasite genera contributing to infection. The intensity of lectin staining varied with incubation time, incubation volume, concentration of lectin and concentration of eggs. Formalin fixed eggs had greater autofluorescence but exhibited the same lectin staining pattern as fresh eggs. The stage of egg development did not influence staining. Eggs from Haemonchus contortus, H. placei, Trichostrongylus colubriformis, T. vitrinus, Ostertagia circumcincta, Nematodirus spathiger and the cestode Monezia expansa were incubated with a panel of fluoroscein isothiocyanate (FITC)-labelled lectins. Lectin binding exhibited a genus specific pattern. Haemonchus spp. stained strongly positive with peanut agglutinin (PNA), and were positive for concanavalin A (ConA), Ricinus communis agglutinin (RCA) and Maclura pomifera lectin (MPA). Trichostrongylus spp. were PNA-, ConA-, RCA- and strongly MPA+. O. circumcincta were weakly positive for PNA, MPA, ConA and negative for RCA. N. spath...Continue Reading

References

May 1, 1989·International Journal for Parasitology·A F Bird, B M Zuckerman
Sep 1, 1989·International Journal for Parasitology·U R RaoA C Vickery
Jun 1, 1995·International Journal for Parasitology·L F Le JambreE Lacey
Jan 1, 1996·Parasitology Research·D KerboeufD Soubieux

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Citations

Dec 7, 2007·PLoS Neglected Tropical Diseases·Candida Fagundes TeixeiraCarlos Graeff-Teixeira
Oct 4, 2005·International Journal for Parasitology·Katrin Hammerschmidt, Joachim Kurtz
Nov 6, 2013·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Abdul JabbarBrown Besier
Sep 27, 2014·Journal of Immunology Research·Sarah Jane Margaret PrestonDavid Piedrafita
Apr 19, 2021·Veterinary Parasitology·Ibrahim AbbasMichael B Hildreth

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