Use of LUCS (Light-Up Cell System) as an alternative live cell method to predict human acute oral toxicity

Toxicology Reports
Camille GirondeChristophe Furger

Abstract

LUCS (Light-Up Cell System) is a new live cell test that allows assessment of a cell's homeostasis and its alteration by a toxic agent. To evaluate the effectiveness of LUCS as an alternative test method for acute oral toxicity, we compared EC50s determined in HepG2 cells treated with 53 chemicals selected from the ACuteTox EU database with corresponding human blood LC50s derived from human acute poisoning cases. Linear regression analysis showed that LUCS results predict human data to 69 %. Rodent oral LD50s and LUCS EC50s were then correlated to human LC50s using shared data sets. Linear regression analyses comparing LUCS and animal data clearly showed that LUCS always predicts human toxicity better than animal data do. These successful prediction values prompted us to simplify the LUCS test, adapting it to regulatory and high throughput applications, resulting in a new protocol with consistent dose-response profiles and EC50s. This study demonstrates that the LUCS test method could be relevant for assessing human acute oral toxicity with a simplified protocol adapted to commercially available fluorescence readers. We suggest that this new alternative method can be used for acute systemic toxicity testing in combination with ...Continue Reading

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Citations

Apr 10, 2021·Journal of Toxicology and Environmental Health. Part B, Critical Reviews·Kyu-Bong KimByung-Mu Lee

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Methods Mentioned

BETA
in
biosensors

Software Mentioned

ACuteTox
MEIC
ODCE
GraphPad Prism
REACH

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