Use of mannosylated cationic liposomes/ immunostimulatory CpG DNA complex for effective inhibition of peritoneal dissemination in mice

The Journal of Gene Medicine
Yukari KuramotoMitsuru Hashida

Abstract

Immunotherapy using immunostimulatory CpG DNA could be a promising new therapeutic approach to combat refractory peritoneal dissemination. In the present study, we report the use of a mannosylated cationic liposomes/immunostimulatory CpG DNA complex (Man/CpG DNA lipoplex) for effective inhibition of peritoneal dissemination in mice. The immune response characteristics of the Man/CpG DNA lipoplex were evaluated by measuring tumor necrosis factor (TNF)-alpha production using primary cultured mouse peritoneal macrophages. Subsequently, Man/CpG DNA lipoplex was administered intraperitoneally (i.p.) to peritoneal dissemination model mice, and the number of tumor cells (colon26/Luc) was quantitatively evaluated by measuring luciferase activity. The effect on survival time of the Man/CpG DNA lipoplex was also investigated. The serum transaminase levels of mice receiving i.p. Man/CpG DNA lipoplex treatment were measured to evaluate systemic toxicity. The Man/CpG DNA lipoplex induced higher TNF-alpha production from macrophages than CpG DNA complexed with conventional cationic liposomes and galactosylated cationic liposomes (Bare/CpG DNA lipoplex and Gal/CpG DNA lipoplex), suggesting mannose receptor-mediated CpG DNA transfer. Intraperi...Continue Reading

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Citations

Dec 2, 2008·Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·Shigeru Kawakami
May 24, 2012·International Journal of Nanomedicine·Nobutaka Hanagata
Jan 18, 2013·Therapeutic Delivery·Joanne M RamseySally-Ann Cryan
Oct 14, 2010·Molecular Membrane Biology·Sylvia A KuijpersRaymond M Schiffelers
Oct 16, 2013·Expert Review of Vaccines·Francesca UngaroLuigi Buonaguro
Jun 6, 2008·Expert Opinion on Drug Delivery·Juan M IracheSocorro Espuelas
Jun 11, 2014·Journal of Controlled Release : Official Journal of the Controlled Release Society·Shigeru Kawakami, Mitsuru Hashida
Mar 9, 2012·Biomaterials·Keerti JainNarendra K Jain
Apr 11, 2009·Trends in Pharmacological Sciences·Mary K O'Reilly, James C Paulson
Sep 9, 2008·International Journal of Pharmaceutics·Raymond M Schiffelers, Gert Storm
Apr 23, 2011·Journal of Drug Delivery·Ciara KellySally-Ann Cryan
May 7, 2010·Clinical and Translational Science·David M Mirvis

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