Use of massively parallel pyrosequencing to evaluate the diversity of and selection on Plasmodium falciparum csp T-cell epitopes in Lilongwe, Malawi.

The Journal of Infectious Diseases
Jeffrey A BaileyJonathan J Juliano

Abstract

The development of an effective malaria vaccine has been hampered by the genetic diversity of commonly used target antigens. This diversity has led to concerns about allele-specific immunity limiting the effectiveness of vaccines. Despite extensive genetic diversity of circumsporozoite protein (CS), the most successful malaria vaccine is RTS/S, a monovalent CS vaccine. By use of massively parallel pyrosequencing, we evaluated the diversity of CS haplotypes across the T-cell epitopes in parasites from Lilongwe, Malawi. We identified 57 unique parasite haplotypes from 100 participants. By use of ecological and molecular indexes of diversity, we saw no difference in the diversity of CS haplotypes between adults and children. We saw evidence of weak variant-specific selection within this region of CS, suggesting naturally acquired immunity does induce variant-specific selection on CS. Therefore, the impact of CS vaccines on variant frequencies with widespread implementation of vaccination requires further study.

References

Nov 1, 1989·The Journal of Psychology·A S Luchins
Jan 1, 1987·International Journal of Aging & Human Development·B McKenzie, J Campbell
Nov 1, 1994·The American Journal of Tropical Medicine and Hygiene·S JongwutiwesA L Hughes
May 20, 1999·Molecular Biology and Evolution·H J BandeltA Röhl
Mar 4, 2000·Molecular and Biochemical Parasitology·A AllouecheB M Greenwood
Oct 12, 2004·Annual Review of Cell and Developmental Biology·Stefan H I KappeVictor Nussenzweig
Jun 1, 2005·Mathematical Biosciences·Mimmo IannelliXue-Zhi Li
Sep 9, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Chutima KumkhaekGeorges Snounou
Oct 19, 2006·International Journal for Parasitology·Gareth D WeedallDavid J Conway
Feb 1, 2007·Journal of the Royal Society, Interface·Sylvain Gandon, Troy Day
Apr 27, 2007·Journal of the Royal Society, Interface·Maia MartchevaRobert D Holt
Jun 20, 2007·Nucleic Acids Research·Christian HoffmannFrederic D Bushman
Apr 30, 2008·Malaria Journal·Vivian N HawkinsCarol Hopkins Sibley
Feb 13, 2009·The Journal of Infectious Diseases·Birgitte B SimenUNKNOWN Terry Beirn Community Programs for Clinical Research on AIDS
Oct 7, 2009·Human Vaccines·Joe CohenAmanda Leach
Jan 15, 2010·Proceedings. Biological Sciences·Lewis G Spurgin, David S Richardson
Jan 23, 2010·Biologicals : Journal of the International Association of Biological Standardization·UNKNOWN WHO
Feb 19, 2010·Science Translational Medicine·Shannon L TakalaChristopher V Plowe
Mar 26, 2010·Interactive Cardiovascular and Thoracic Surgery·Karsten KnoblochPeter M Vogt
Nov 3, 2010·Lancet·Richard G A FeachemRobert W Snow
Nov 3, 2010·Lancet·Pam Das, Richard Horton
Nov 3, 2010·Proceedings of the National Academy of Sciences of the United States of America·Jonathan J JulianoSteven R Meshnick
Apr 28, 2011·BMC Bioinformatics·Osvaldo ZagordiNiko Beerenwinkel
May 14, 2011·Molecular Ecology Resources·Laurent Excoffier, Heidi E L Lischer
Sep 16, 2011·The New England Journal of Medicine·Mahamadou A TheraChristopher V Plowe
Oct 20, 2011·The New England Journal of Medicine·UNKNOWN RTS,S Clinical Trials PartnershipPreeti Vansadia

❮ Previous
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Citations

Jun 12, 2013·Trends in Parasitology·Nicole MideoAndrew F Read
Jan 12, 2010·Chaos·Eric ForgostonIra B Schwartz
Jul 2, 2010·Chaos·Zhenzhen LiuMogei Wang
Jan 10, 2012·Chaos·Kai GongMingsheng Shang
Apr 20, 2014·PLoS Neglected Tropical Diseases·Christian M ParobekJonathan J Juliano
Jun 20, 2014·PLoS Computational Biology·Daniel J LaydonBecca Asquith
Jun 21, 2014·The Journal of Infectious Diseases·Harold OchollaJacqui Montgomery
Aug 15, 2014·Frontiers in Immunology·Alyssa E Barry, Alicia Arnott
Nov 24, 2017·The Journal of Experimental Medicine·Stephen W ScallyJean-Philippe Julien
Apr 22, 2015·Pathogens and Global Health·Rachel F DanielsDaniel L Hartl
Sep 3, 2019·Current Pharmaceutical Design·Brijesh S YadavNinoslav Marina
Sep 3, 2013·Archives of Disease in Childhood·Mercy MulakuMike English
Oct 22, 2015·The New England Journal of Medicine·D E NeafseyD F Wirth
Jan 29, 2016·Evolution, Medicine, and Public Health·Nicole MideoJonathan J Juliano
Apr 9, 2017·Cold Spring Harbor Perspectives in Medicine·Daniel E Neafsey, Sarah K Volkman
Jan 8, 2019·Human Vaccines & Immunotherapeutics·Sundos KhanAnne S De Groot
Apr 13, 2018·Nature Communications·Angela M EarlyDaniel E Neafsey
Mar 29, 2016·PLoS Neglected Tropical Diseases·Lindsey R FriedrichDavid Serre
Apr 10, 2021·Nature Reviews. Genetics·Daniel E NeafseyBronwyn L MacInnis
Aug 10, 2021·Current Epidemiology Reports·Griffin J BellJeffrey A Bailey

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