Use of metallochromic dyes to measure changes in myoplasmic calcium during activity in frog skeletal muscle fibres

The Journal of Physiology
S M BaylorM W Marshall

Abstract

1. Changes in transmission of quasi-monochromatic light were measured in singly dissected, dye-injected twitch fibres following a single propagated action potential. The records, after correction for the intrinsic transmission signal, indicate changes in dye-related absorbance, DeltaA. This paper describes the different components of dye-related signals in fibres injected with either Arsenazo III, Antipyrylazo III or Dichlorophosphonazo III.2. Fibres injected with Arsenazo III can show two kinds of changes in dye-related absorbance, an early isotropic change and a later dichroic change. The isotropic signal, which is the main subject of this paper, is transient in nature; it starts to develop before tension, reaches a peak in about 10 msec and is nearly over by 0.1 sec (16 degrees C). This signal is largest at 650-660 nm and measurements in this range indicate that the peak DeltaA varies approximately linearly with dye concentration between 0.2 and 0.7 mM. The wavelength dependence of the peak amplitude can be qualitatively fitted by the Ca(2+)-difference spectrum determined from cuvette calibration measurements. There may be a small maintained (0.4-0.5 sec) absorbance change of a few percent of the peak value at 650-660 nm, po...Continue Reading

Citations

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