PMID: 9446821Apr 4, 1998Paper

Use of methacrylate De-embedding protocols for in situ hybridization on semithin plastic sections with multiple detection strategies

The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society
K C WarrenS C Cary

Abstract

New plastic resins are gradually replacing traditional paraffin-embedding in situ hybridization (ISH) strategies. One unique resin that has not been fully investigated or exploited with respect to light microscopic ISH is a methacrylate mixture. The advantage of this resin is its ability to be removed from tissues postsectioning, dramatically increasing hybridization signal compared to that obtained in other plastics. The goal of this study was to evaluate the general applicability of the methacrylate embedding acetone de-embedding (MEADE) technique for ISH investigations. Several high-resolution, high-sensitivity ISH protocols are described, using both end-labeled oligonucleotides and randomly primed DNA probes (200-400 BPS), signal amplification by catalyzed reporter deposition (CARD), and chromogenic and fluorescent detection methods. With slight modification, the MEADE ISH technique permitted localization of bacterial symbionts in fragile gill tissue and collagen transcripts in foot tissue of two marine bivalves. MEADE ISH has proved extremely versatile and will likely be suitable for many future applications.

References

Nov 1, 1992·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·R W DirksA K Raap
Aug 1, 1991·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·M P Wenderoth, B R Eisenberg
Feb 1, 1986·The American Journal of Anatomy·B G Timms
Sep 1, 1987·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·E KellenbergerM Wurtz
Mar 1, 1987·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·P J Harris, B P Rubin
Apr 1, 1995·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·H M KerstensA G Hanselaar

❮ Previous
Next ❯

Citations

Sep 19, 2000·Journal of Microbiological Methods·A Moter, U B Göbel
Apr 1, 2000·Applied and Environmental Microbiology·A R SipeS C Cary
May 14, 2009·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Jacob S TorgersenOivind Andersen
Jun 15, 2006·Journal of Neuropathology and Experimental Neurology·Marcus MüllerReinhard Kiefer
Sep 11, 2007·The Plant Journal : for Cell and Molecular Biology·Sabine GubatzUlrich Wobus
Apr 5, 2005·Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission·A L SawitzkeG C Schoenwolf
Apr 28, 2004·Developmental Biology·Chiawei W TsaiStephen M Wolniak
Apr 29, 1999·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·J C AlersH van Dekken
Oct 18, 2005·The Journal of Biological Chemistry·Hua ZhaoJ Herbert Waite

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.