Use of molecular subtyping methods to investigate two nosocomial outbreaks due to Salmonella Livingstone in Sfax hospital, Tunisia

Pathologie-biologie
Sonia KtariA Hammami

Abstract

The aim of the study was to investigate two nosocomial outbreaks due to Salmonella Livingstone in a pediatric ward in Sfax hospital using molecular typing techniques. We included 84 strains of S. Livingstone isolated from patients hospitalized in a pediatric ward between November 1999 through August 2002 in addition to one environmental sample. Three epidemiological unrelated strains of S. Livingstone were also tested. The molecular typing techniques were: plasmid analysis, enterobacterial repetitive intergenic consensus (ERIC-PCR), random amplification of polymorphic DNA (RAPD-PCR) and pulsed field gel electrophoresis (PFGE). The plasmid analysis and the ERIC-PCR generated a similar profile for outbreak isolates including the environmental sample while the epidemiologically unrelated strains demonstrated distinct patterns. The RAPD-PCR applied on 20 strains showed three patterns but one profile was predominating. All the strains isolate of S. Livingstone, except the veterinary strain, could not be typed by PFGE. Using the molecular typing techniques, we showed that these two outbreaks in the pediatric ward were due to the clonal spread of a single strain of S. Livingstone. The identification of the source of contamination and ...Continue Reading

References

Jun 1, 1996·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·D L BaggesenJ P Christensen
Sep 28, 2001·Journal of Clinical Microbiology·E LiebanaR H Davies
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Citations

Oct 28, 2009·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Sonia KtariAdnane Hammami
Jul 11, 2013·World Journal of Microbiology & Biotechnology·Yousra TurkiHadda Ouzari
Aug 21, 2007·The Journal of Infection·Ridha Ben AissaAbderazzaque Belhadj
Nov 27, 2015·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Sonia KtariAdnene Hammami
Oct 9, 2020·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·Boutheina KsibiAdnene Hammami
Jan 15, 2008·Médecine et maladies infectieuses·D MkaouarA Hammami

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