Jul 3, 2002

Use of multiepitope polyproteins in serodiagnosis of active tuberculosis

Clinical and Diagnostic Laboratory Immunology
Raymond HoughtonSteven G Reed

Abstract

Screening of genomic expression libraries from Mycobacterium tuberculosis with sera from tuberculosis (TB) patients or rabbit antiserum to M. tuberculosis led to the identification of novel antigens capable of detecting specific antibodies to M. tuberculosis. Three antigens, Mtb11 (also known as CFP-10), Mtb8, and Mtb48, were tested together with the previously reported 38-kDa protein, in an enzyme-linked immunosorbent assay (ELISA) to detect antibodies in TB patients. These four proteins were also produced as a genetically fused polyprotein, which was tested with two additional antigens, DPEP (also known as MPT32) and Mtb81. Sera from individuals with pulmonary and extrapulmonary TB, human immunodeficiency virus (HIV)-TB coinfections, and purified protein derivative (PPD)-positive and PPD-negative status with no evidence of disease were tested. In samples from HIV-negative individuals, the ELISA detected antibodies in >80% of smear-positive individuals and >60% smear-negative individuals, with a specificity of approximately 98%. For this group, smears detected 81.6% but a combination of smear and ELISA had a sensitivity of approximately 93%. The antigen combination detected a significant number of HIV-TB coinfections as well a...Continue Reading

  • References19
  • Citations58

References

Mentioned in this Paper

3-(4-((2,6-dichloropyridin-4-yl)ethynyl)phenyl)propanoic acid
Polyproteins
Procedure to Identify Antibody
Mtb81 protein, Mycobacterium tuberculosis
Lung
Genome
Tuberculosis
Chimeric Proteins, Recombinant
Serodiagnosis
Tuberculosis, Meningeal

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