Use of multiparameter analysis for Vibrio alginolyticus viable but nonculturable state determination

Cytometry. Part a : the Journal of the International Society for Analytical Cytology
Maria Cristina AlbertiniBarbara Citterio

Abstract

Vibrio alginolyticus is known to enter into a viable but nonculturable (VBNC) state in response to environmental conditions unfavorable to the growth. Cells in VBNC condition pose a public health threat because they are potentially pathogenic. We constructed a pathway for the identification of the most significant variables and the characterization of those variables able to discriminate the groups under investigation. Different parameters measured by the image processing software were chosen as the most representative of V. alginolyticus cell morphology (length index for dimension) and metabolic activity (density profile indexes). To detect relationships between the groups of treatment performed, we carried out a principal components analysis (PCA). The PCA analysis indicated that increasing coccoid shape transformation was related to both metabolic and dimension variations, delineating a well defined graph profile. Indeed, we discovered that specific morphological variations occur when cells in the culturable state pass into VBNC condition, namely comma-shaped culturable bacteria are converted into coccoid-shaped VBNC cells. The results were also supported by scanning electron microscopy analysis. This technique allows the an...Continue Reading

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Citations

Jun 15, 2007·Journal of Microbiological Methods·Susanne GüntherSusann Müller
Dec 7, 2007·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Susann Müller
Jun 3, 2009·Journal of Applied Microbiology·U P Cronin, M G Wilkinson
Mar 27, 2010·FEMS Microbiology Reviews·Susann Müller, Gerhard Nebe-von-Caron
Jan 25, 2012·Computational and Mathematical Methods in Medicine·Shengyong ChenJianwei Zhang
Jan 8, 2013·International Journal of Food Microbiology·Chao-Ping SuHin-chung Wong
Apr 11, 2007·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Lei ShiSusann Müller
Feb 3, 2007·Cytometry. Part B, Clinical Cytometry·Laura TeodoriMaria Cristina Albertini
Apr 20, 2017·Frontiers in Microbiology·Xihong ZhaoTian Ding
Apr 15, 2015·Archives of Microbiology·Barbara CitterioElena Piatti
Dec 16, 2020·Comprehensive Reviews in Food Science and Food Safety·Kai DongXiaojun Liao

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