Use of multiplexed tandem PCR to estimate the prevalence and intensity of Theileria orientalis infections in cattle

Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases
Piyumali K PereraAbdul Jabbar

Abstract

This study employed a semi-quantitative, multiplexed tandem PCR (MT-PCR) to assess the prevalence and infection intensity of four genotypes (buffeli, chitose, ikeda and type 5) of Theileria orientalis in cattle in Australia. Genomic DNA samples from blood samples (n=448) collected from 27 to 32 dairy cows from each of 15 dairy herds with a history of recent theileriosis outbreaks (Group 1), and from blood samples available from 24 cows with or without oriental theileriosis (Group 2) were tested using MT-PCR. Results revealed that all four genotypes were present in Group 1 cattle; genotype buffeli had the highest prevalence (80.5%), followed by genotypes ikeda (71.4%), chitose (38.6%) and type 5 (20.3%). Genotype ikeda had the highest average infection intensity in the cattle (relating to 55,277 DNA copies), followed by buffeli, chitose and type 5 (6354-51,648 copies). For Group 2, results indicated that genotype ikeda had a significantly higher average intensity of infection than buffeli in symptomatic cattle (P<0.001), and symptomatic cattle had a higher intensity of ikeda than asymptomatic cattle (P=0.004). Future studies should assess the utility of the present MT-PCR assay as a diagnostic and epidemiological tool in other p...Continue Reading

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Feb 21, 2014·Parasites & Vectors·Piyumali K PereraAbdul Jabbar
Aug 8, 2014·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Thillaiampalam SivakumarNaoaki Yokoyama

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Citations

Apr 2, 2016·Ticks and Tick-borne Diseases·Hagos GebrekidanAbdul Jabbar
Nov 14, 2016·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Hagos GebrekidanAbdul Jabbar
Oct 29, 2019·The Journal of Clinical Pediatric Dentistry·Sigalit BlumerBenjamin Peretz
Jun 27, 2017·The Journal of Clinical Pediatric Dentistry·Sigalit BlumerBenjamin Peretz

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