PMID: 2094593Jan 1, 1990Paper

Use of non-human primate hepatocytes for in vitro study of the pre-erythrocytic stages of malaria parasites

Bulletin of the World Health Organization
P MilletP Nguyen-Dinh

Abstract

Methods were developed that allow invasion of sporozoites from simian malaria parasite species (Plasmodium cynomolgi, P. knowlesi, P. coatneyi, P. inui, P. gonderi, P. fragile) and development to schizont stages in rhesus and Saimiri monkey hepatocytes. The P. cynomolgi-rhesus monkey model was used to study inhibition of schizont development using monoclonal antibodies (MAbs) produced against the circumsporozoite (CS) protein of various strains and species of malaria parasites. Immunoelectron microscopy, using gold-labelled MAbs and cultured parasites, demonstrated that the CS protein persists in 7-day old liver stages of P. cynomolgi, but is not expressed at the surface of infected hepatocytes. A rhesus monkey was immunized with autologous hepatocytes (collected by biopsy) infected in vitro with liver stages of P. cynomolgi. This immunization elicited antibodies reacting with sporozoite, liver stage, and blood-stage parasites. In addition, human malaria parasites (P. falciparum, P. vivax, P. malariae) have been cultured in Saimiri or rhesus monkey hepatocytes. The P. vivax-Saimiri monkey model was used to study inhibition activity of sera from Saimiri monkeys experimentally immunized with recombinant P. vivax CS proteins. Post...Continue Reading

Related Concepts

Related Feeds

Antimalarial Agents (ASM)

Antimalarial agents, also known as antimalarials, are designed to prevent or cure malaria. Discover the latest research on antimalarial agents here.

Antimalarial Agents

Antimalarial agents, also known as antimalarials, are designed to prevent or cure malaria. Discover the latest research on antimalarial agents here.