PMID: 16511789Mar 3, 2006Paper

Use of Oil Red O stain in the cytologic diagnosis of canine liposarcoma

Veterinary Clinical Pathology
Carlo MasserdottiNicola Ottolini

Abstract

Oil Red O, a stain commonly used to demonstrate lipid in frozen tissue, also may be used to stain air-dried cytologic specimens. The purpose of this study was to prospectively evaluate the value of Oil Red O in identifying lipid to aid in the differentiation of liposarcomas from other types of sarcoma. Twelve tumor specimens from dogs were evaluated. The tumors were included in the study if initial cytologic evaluation indicated a sarcoma, and if histologic confirmation was available. Oil Red O was applied to all cytologic specimens. Tumor specimens were diagnosed histologically as liposarcoma (3 well-differentiated, 1 pleomorphic), hemangiopericytoma (n = 3), fibrosarcoma (n = 3), malignant fibrous histiocytoma (n = 1), and undifferentiated sarcoma (n = 1). Cytologic specimens from all liposarcomas showed strong positive staining of cytoplasmic vacuoles for lipid. Specimens from other sarcomas stained negative for Oil Red O, with the exception of weak, irregular positive staining in 1 hemangiopericytoma. Our results suggest that Oil Red O staining may be an easy, inexpensive, and useful diagnostic tool for the differentiation of liposarcoma from other mesenchymal neoplasms.

Citations

Aug 19, 2011·Veterinary Clinical Pathology·Eleonora PisedduCarlo Masserdotti
Feb 9, 2012·Journal of the American Veterinary Medical Association·Anh N Diep, Rebekah I Fleis
May 21, 2011·Topics in Companion Animal Medicine·Amy L MacNeill
Jan 24, 2015·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Georgina Doria-TorraEnric Vidal
Oct 6, 2009·American Journal of Physiology. Heart and Circulatory Physiology·Ratnadeep BasuZamaneh Kassiri
Nov 26, 2019·Veterinary Clinical Pathology·Elise B Russell, Natalie F Courtman
Jan 17, 2020·The Journal of Small Animal Practice·J Pérez-AccinoP Cazzini
Jan 10, 2020·Veterinary Clinical Pathology·Camille A McAloneyJessica A Hokamp

❮ Previous
Next ❯

Related Concepts

Related Feeds

Carcinosarcoma

Carcinosarcoma is a malignant neoplasm that contains elements of carcinoma and sarcoma so extensively intermixed as to indicate neoplasia of epithelial and mesenchymal tissue. Discover the latest research on carcinosarcoma here.