Use of oligoarrays for characterization of community-onset methicillin-resistant Staphylococcus aureus

Journal of Clinical Microbiology
Thibaud KoesslerJacques Schrenzel

Abstract

Until recently, methicillin-resistant Staphylococcus aureus (MRSA) was considered the prototype of a hospital-acquired bacterial pathogen. However, recent reports have shown that MRSA has now emerged in the community. Characterization of specific markers for distinguishing the origin of isolates could contribute to improved knowledge of MRSA epidemiology. The release of whole-genome sequences of hospital- and community-acquired S. aureus strains allowed the development of whole-genome content analysis techniques, including microarrays. We developed a microarray composed of 8,191 open reading frame-specific oligonucleotides covering >99% of the four sequenced S. aureus genomes (N315, Mu50, MW2, and COL) to evaluate gene contents of hospital- and community-onset S. aureus strains. In parallel, pulsed-field gel electrophoresis, variable number of tandem repeats, antibiogram, staphylococcal cassette chromosome-mec element typing, and presence of the Panton-Valentine leukocidin gene were evaluated in a collection of 15 clinical isolates. Clusters obtained with microarrays showed a high degree of similarity with those obtained by pulsed-field gel electrophoresis or variable number of tandem repeats. Clusters clearly segregated hospit...Continue Reading

References

Dec 1, 1986·European Journal of Clinical Microbiology·J H Jorgensen
Sep 1, 1995·Journal of Clinical Microbiology·L A TeixeiraA Tomasz
May 23, 1998·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·G L Archer
Dec 20, 2000·Proceedings of the National Academy of Sciences of the United States of America·N SalamaS Falkow
Feb 13, 2001·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·A DeplanoM J Struelens
Jul 12, 2001·Proceedings of the National Academy of Sciences of the United States of America·J R FitzgeraldJ M Musser
Aug 31, 2001·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·T S NaimiK E Smith
Aug 1, 1996·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·M. J. Struelens
May 29, 2002·Proceedings of the National Academy of Sciences of the United States of America·Mark C EnrightBrian G Spratt
Jun 5, 2002·Lancet·Tadashi BabaKeiichi Hiramatsu
Aug 30, 2002·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·D S BlancP Francioli
Sep 14, 2002·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Philippe DufourHervé Richet
Oct 18, 2002·Nucleic Acids Research·Adel M TalaatStephen Albert Johnston
Nov 1, 2002·Journal of Clinical Microbiology·Keiko OkumaKeiichi Hiramatsu
Dec 25, 2002·Antimicrobial Agents and Chemotherapy·P D FeyP M Schlievert
Jun 12, 2003·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·D S BlancP M Hauser
Jun 28, 2003·Infection Control and Hospital Epidemiology : the Official Journal of the Society of Hospital Epidemiologists of America·Battouli Saïd-SalimBarry N Kreiswirth
Aug 30, 2003·Archives of Pathology & Laboratory Medicine·Najam ZaidiMarcus Zervos
Dec 11, 2003·JAMA : the Journal of the American Medical Association·Timothy S NaimiRuth Lynfield
Feb 28, 2004·Bioinformatics·Robert KerkhovenRoland J Siezen
Jun 16, 2004·Current Opinion in Microbiology·Edward J Feil, Mark C Enright
Jun 19, 2004·Journal of Bacteriology·Markus BischoffSteven Projan
Jun 19, 2004·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Edwin D CharleboisHenry F Chambers
Jul 10, 2004·Journal of Clinical Microbiology·Patrice FrancoisJacques Schrenzel
Aug 5, 2004·Journal of Bacteriology·Tsute ChenMargaret J Duncan
Oct 9, 2004·Journal of Clinical Microbiology·Geoffrey W CoombsUNKNOWN Australian Group for Antimicrobial Resistance
Dec 8, 2004·Journal of Clinical Microbiology·Vincent RamisseGilles Vergnaud
Feb 4, 2005·The New England Journal of Medicine·Sophia V KazakovaDaniel B Jernigan

❮ Previous
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Citations

Jul 30, 2008·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·D C MellesJ Schrenzel
Jan 24, 2007·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Ulrich Seybold, Henry M Blumberg
Jun 2, 2011·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Andie S LeeStephan Harbarth
May 12, 2009·Applied and Environmental Microbiology·Sergine EvenYves Le Loir
Oct 14, 2009·Clinical Microbiology Reviews·Melissa B Miller, Yi-Wei Tang
Jun 24, 2008·Journal of Bacteriology·Nouri L Ben ZakourYves Le Loir
May 20, 2009·BMC Microbiology·Kati SeidlBrigitte Berger-Bächi
May 19, 2011·Future Microbiology·Vladimir LazarevicPatrice François
Aug 12, 2014·The Journal of Antimicrobial Chemotherapy·Anne BerscheidGabriele Bierbaum
Nov 4, 2009·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Gerrit KuhnDominique S Blanc
Nov 3, 2009·Journal of Proteomics·Patrice FrançoisJacques Schrenzel
Mar 26, 2009·Journal of Microbiological Methods·Adrien FischerJacques Schrenzel
Nov 23, 2006·Journal of Microbiological Methods·Patrice FrancoisJacques Schrenzel
Dec 17, 2009·International Journal of Medical Microbiology : IJMM·Marie BeaumeJacques Schrenzel
Feb 22, 2012·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Christopher D SibleyDeirdre L Church
Oct 15, 2008·Proteome Science·Emilie ErnoultCatherine Guette
Oct 9, 2012·Journal of Microbiological Methods·Cynthia E ZimmermanDarrell P Chandler
May 7, 2009·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·Y LongtinS Harbarth
Oct 23, 2009·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·C MégevandUNKNOWN Paediatric Infectious Disease Group Switzerland Staphylococcus aureus Study Group
Apr 6, 2012·PloS One·Amrita LamaMichiko M Nakano
Nov 11, 2008·PloS One·Patricia RenestoDidier Raoult
Nov 19, 2011·PloS One·Caroline Le MaréchalYves Le Loir
Oct 17, 2016·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·D O AndreyA Renzoni
Aug 15, 2014·Journal of Clinical Microbiology·Andie S LeePatrice François

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