Use of oligonucleotide microarrays for rapid detection and serotyping of acute respiratory disease-associated adenoviruses

Journal of Clinical Microbiology
Baochuan LinDavid A Stenger

Abstract

The cessation of the adenovirus vaccination program for military trainees has resulted in several recent acute respiratory disease (ARD) outbreaks. In the absence of vaccination, rapid detection methods are necessary for the timely implementation of measures to prevent adenovirus transmission within military training facilities. To this end, we have combined a fluorogenic real-time multiplex PCR assay with four sets of degenerate PCR primers that target the E1A, fiber, and hexon genes with a long oligonucleotide microarray capable of identifying the most common adenovirus serotypes associated with adult respiratory tract infections (serotypes 3, 4, 7, 16, and 21) and a representative member of adenovirus subgroup C (serotype 6) that is a common cause of childhood ARD and that often persists into adulthood. Analyses with prototype strains demonstrated unique hybridization patterns for representative members of adenovirus subgroups B(1), B(2), C, and E, thus allowing serotype determination. Microarray-based sensitivity assessments revealed lower detection limits (between 1 and 100 genomic copies) for adenovirus serotype 4 (Ad4) and Ad7 cell culture lysates, clinical nasal washes, and throat swabs and purified DNA from clinical sa...Continue Reading

References

Sep 1, 1969·The Journal of General Virology·E Norrby
Oct 1, 1993·Virology·W C GruberC Tibbetts
Sep 1, 1996·Journal of Clinical Microbiology·W Saitoh-InagawaH Ishiko
Mar 1, 1996·Journal of Clinical Microbiology·A H KiddJ C De Jong
May 15, 1999·Journal of Clinical Microbiology·S TakeuchiS Ohno
Oct 3, 1999·Journal of Virological Methods·S K Poddar
Jun 2, 2000·Journal of Clinical Microbiology·E M ElnifroA S Bailey
Oct 6, 2000·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·G C GrayD P Schnurr
Nov 4, 2000·Journal of Clinical Microbiology·W XuD D Erdman
Feb 28, 2001·Journal of Virological Methods·A AvellónJ E Echevarría
Jul 27, 2001·Journal of Medical Virology·W Xu, D D Erdman
Jul 28, 2001·Journal of Clinical Microbiology·M D MalasigG C Gray
Jan 23, 2002·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Margaret A K RyanMarietta D Malasig
Mar 7, 2002·Journal of Clinical Microbiology·Sergey LapaAndrei Mirzabekov
May 15, 2002·Diagnostic Microbiology and Infectious Disease·Huo-Shu H HoungLeonard N Binn
Jun 29, 2002·Journal of Clinical Microbiology·V ChizhikovK Chumakov
Sep 14, 2002·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Shellie A Kolavic-GrayBruce L Innis
Nov 14, 2002·Proceedings of the National Academy of Sciences of the United States of America·David WangJoseph L DeRisi
Mar 15, 2003·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·S MitchellP V Coyle
Apr 12, 2003·Journal of Clinical Microbiology·Kazunori KawaguchiKenichi Kobayashi
Apr 16, 2003·Journal of Medical Virology·Albert HeimPatricia Pring-Akerblom

❮ Previous
Next ❯

Citations

Mar 3, 2011·Current Infectious Disease Reports·Shu ZhangYi-Wei Tang
Oct 30, 2012·The Journal of Microbiology·Ju-Mi KimGwangPyo Ko
Oct 16, 2007·Applied and Environmental Microbiology·Irene XagorarakiJoan B Rose
Feb 5, 2008·Applied and Environmental Microbiology·Sarah M MillerSyed A Hashsham
Oct 14, 2009·Clinical Microbiology Reviews·Melissa B Miller, Yi-Wei Tang
Sep 12, 2008·Journal of Clinical Microbiology·Zhengshan ZhaoMichel G Bergeron
Sep 6, 2008·BMC Molecular Biology·Nicolas BerthetJean-Claude Manuguerra
Feb 11, 2010·Future Microbiology·Tomasz A LeskiBaochuan Lin
May 19, 2006·Emerging Infectious Diseases·Gary J VoraDavid Metzgar
May 7, 2013·Future Microbiology·Sabri BousbiaBernard La Scola
Jul 22, 2011·Veterinary Medicine International·Neeta LongjamV K Saxena
Aug 19, 2008·Diagnostic Microbiology and Infectious Disease·Meei-Li HuangLawrence Corey
Mar 28, 2007·Heart Failure Clinics·Neil E BowlesJeffrey A Towbin
Aug 22, 2006·The New Phytologist·A SessitschL Bodrossy
Aug 2, 2006·Transfusion Medicine·J Petrik
Jan 10, 2009·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Jeong-Weon HuhYoung-Hee Lim
Aug 30, 2005·Clinica Chimica Acta; International Journal of Clinical Chemistry·Alexander Loy, Levente Bodrossy
Oct 31, 2014·Journal of Clinical Microbiology·Miguel A MartínezPavel Isa
Nov 30, 2005·BioTechniques·Concepcion Conejero-GoldbergE Fuller Torrey
Aug 25, 2006·Reviews in Medical Virology·Helen E Ambrose, Jonathan P Clewley
Nov 9, 2018·Frontiers in Microbiology·Shubhagata DasYi-Wei Tang
Jun 14, 2005·Clinical Chemistry·Barbara ChmielewiczHeinz Ellerbrok
May 31, 2016·World Journal of Gastroenterology : WJG·Mohammad IrshadMohammad Ahmad Ansari

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Proceedings of the National Academy of Sciences of the United States of America
David WangJoseph L DeRisi
© 2022 Meta ULC. All rights reserved